PMID- 28409016
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2053-8790 (Print)
IS  - 2053-8790 (Electronic)
IS  - 2053-8790 (Linking)
VI  - 4
IP  - 1
DP  - 2017
TI  - Surface complement C3 fragments and cellular binding of microparticles in 
      patients with SLE.
PG  - e000193
LID - 10.1136/lupus-2016-000193 [doi]
LID - e000193
AB  - OBJECTIVES: To examine microparticles (MPs) from patients with SLE and healthy 
      controls (HCs) by determining the cellular origin of the MPs, quantifying 
      attached fragments of complement component 3 (C3) and assessing the ability of 
      MPs to bind to circulating phagocytes and erythrocytes. These features may be 
      relevant for clearance of MPs in SLE pathogenesis. METHODS: Attached C3 fragments 
      (C3b, iC3b, C3d), membrane integrity and cell surface markers of MPs from 18 
      patients with SLE and 11 HCs were measured by adding specific antibodies, 
      7-aminoactinomycin D (7AAD) and annexin V. MPs from all subjects were labelled 
      with carboxyfluorescein diacetate succinimidyl ester and allowed to bind to 
      autologous phagocytes and erythrocytes in the presence of autologous serum, and 
      the binding to individual cell populations was assessed by flow cytometry. 
      RESULTS: The proportion of MPs bearing C3 fragments was higher in patients with 
      SLE than in HCs (p=0.026), but the amount of opsonising C3b/iC3b molecules was 
      lower (p=0.004). The C3b/iC3b level correlated with the concentration of 
      circulating C3 (r(s)=0.53, p=0.036). Phagocytes and erythrocytes from patients 
      and HCs bound autologous MPs, and granulocytes from patients bound 13% more MPs 
      than those from HCs (p=0.043). The presence of erythrocytes inhibited the MP 
      binding to granulocytes by approximately 50%. CONCLUSIONS: Our demonstration of 
      altered composition of C3 fragments on MPs from patients with SLE, including 
      decreased numbers of opsonising C3 fragments, and competitive binding of MPs to 
      circulating phagocytes and erythrocytes corroborates the hypothesis of defective 
      clearance of apoptotic material in SLE, and indicates that differences in both MP 
      opsonisation and binding of MPs to cells are important in the pathogenesis of 
      SLE.
FAU - Winberg, Line Kjaer
AU  - Winberg LK
AD  - Institute for Inflammation Research and Copenhagen Lupus and Vasculitis Clinic, 
      Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
FAU - Nielsen, Claus Henrik
AU  - Nielsen CH
AD  - Institute for Inflammation Research and Copenhagen Lupus and Vasculitis Clinic, 
      Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
FAU - Jacobsen, Soren
AU  - Jacobsen S
AD  - Institute for Inflammation Research and Copenhagen Lupus and Vasculitis Clinic, 
      Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, 
      Rigshospitalet, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20170331
PL  - England
TA  - Lupus Sci Med
JT  - Lupus science & medicine
JID - 101633705
PMC - PMC5387967
OTO - NOTNLM
OT  - Apoptosis
OT  - Autoimmunity
OT  - Complement
OT  - Microparticles
OT  - Systemic Lupus Erythematosus
COIS- Competing interests: None declared.
EDAT- 2017/04/15 06:00
MHDA- 2017/04/15 06:01
PMCR- 2017/03/31
CRDT- 2017/04/15 06:00
PHST- 2016/12/05 00:00 [received]
PHST- 2017/02/16 00:00 [revised]
PHST- 2017/03/11 00:00 [accepted]
PHST- 2017/04/15 06:00 [entrez]
PHST- 2017/04/15 06:00 [pubmed]
PHST- 2017/04/15 06:01 [medline]
PHST- 2017/03/31 00:00 [pmc-release]
AID - lupus-2016-000193 [pii]
AID - 10.1136/lupus-2016-000193 [doi]
PST - epublish
SO  - Lupus Sci Med. 2017 Mar 31;4(1):e000193. doi: 10.1136/lupus-2016-000193. 
      eCollection 2017.