PMID- 28409326
OWN - NLM
STAT- MEDLINE
DCOM- 20180202
LR  - 20181113
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 48
IP  - 3
DP  - 2017 Jun
TI  - Effect of corticision on orthodontic tooth movement in a rat model as assessed by 
      RNA sequencing.
PG  - 199-208
LID - 10.1007/s10735-017-9718-3 [doi]
AB  - Corticision is a common technique to accelerate orthodontic tooth movement; 
      however, not much is known about the underlying mechanisms. In this study, we 
      investigated the mechanism of alveolar tissue remodeling after corticision in a 
      rat model of tooth movement (TM) by analyzing the differential transcriptome. A 
      total of 36 male rats were equally divided into TM and TM with corticision (TM+C) 
      groups. Alveolar bone response was examined using micro-computed tomography 
      (micro-CT). Osteoclasts and osteoblasts were quantified on tartrate-resistant 
      acid phosphatase (TRAP) and Goldner's trichrome staining. The transcriptomes of 
      alveolus around the left maxillary first molar were determined on RNA sequencing 
      (RNA-Seq), and the expression of selected differentially expressed genes (DEGs) 
      validated on quantitative reverse-transcriptase polymerase chain reaction 
      (qRT-PCR). Immunohistochemical examination of alveolar tissue was performed to 
      examine the expressions of correlative proteins of the selected signaling pathway 
      in the TM and TM+C groups. The ratio of bone volume to total volume (BV/TV), and 
      the trabecular number (Tb.N) were significantly decreased, while the movement 
      distance and the trabecular separation (Tb.Sp) was significantly increased in the 
      TM+C group. However, no significant between-group difference in trabecular 
      thickness (Tb.Th) was observed. On histomorphometric analysis, a significant 
      increase in the number of osteoclasts and increased bone resorption was observed 
      in the TM+C group. A total of 399 DEGs were identified on RNA-SEq. Eleven 
      selected genes were confirmed on qRT-PCR, which included components of the Ras 
      signaling pathway. Four proteins of the Ras signaling pathway showed a higher 
      expression in the TM+C group. Our findings indicate that corticision may speed up 
      orthodontic tooth movement by accelerating osteoclastogenesis mediated via the 
      Ras signaling pathway.
FAU - Gu, Qihui
AU  - Gu Q
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China.
FAU - Guo, Shuyu
AU  - Guo S
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China.
FAU - Wang, Dongyue
AU  - Wang D
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China.
FAU - Zhou, Tingting
AU  - Zhou T
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China.
FAU - Wang, Lin
AU  - Wang L
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China.
FAU - Wang, Zhendong
AU  - Wang Z
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China. wangzhd74@hotmail.com.
FAU - Ma, Junqing
AU  - Ma J
AD  - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136 Hanzhong 
      Road, Nanjing, Jiangsu, 210029, China. jma@njmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Alveolar Process
MH  - Animals
MH  - Bone Remodeling/*genetics
MH  - Gene Expression Regulation
MH  - Male
MH  - Osteoclasts
MH  - Rats
MH  - *Sequence Analysis, RNA
MH  - *Tooth Movement Techniques
MH  - Transcriptome/*genetics
MH  - X-Ray Microtomography
MH  - ras Proteins/metabolism
OTO - NOTNLM
OT  - Corticision
OT  - Orthodontic tooth movement
OT  - Osteoclast
OT  - Ras signaling pathway
EDAT- 2017/04/15 06:00
MHDA- 2018/02/03 06:00
CRDT- 2017/04/15 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/03/20 00:00 [accepted]
PHST- 2017/04/15 06:00 [pubmed]
PHST- 2018/02/03 06:00 [medline]
PHST- 2017/04/15 06:00 [entrez]
AID - 10.1007/s10735-017-9718-3 [pii]
AID - 10.1007/s10735-017-9718-3 [doi]
PST - ppublish
SO  - J Mol Histol. 2017 Jun;48(3):199-208. doi: 10.1007/s10735-017-9718-3. Epub 2017 
      Apr 13.