PMID- 28411814
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20180828
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 169
DP  - 2017 Jul 1
TI  - Nanocoating cellulose paper based microextraction combined with nanospray mass 
      spectrometry for rapid and facile quantitation of ribonucleosides in human urine.
PG  - 209-215
LID - S0039-9140(17)30377-6 [pii]
LID - 10.1016/j.talanta.2017.03.085 [doi]
AB  - A rapid and facile analytical method for quantification of ribonucleosides in 
      human urine was developed by the combination of nanocoating cellulose paper based 
      microextraction and nanoelectrospray ionization-tandem mass spectrometry 
      (nESI-MS/MS). Cellulose paper used for microextraction was modified by 
      nano-precision deposition of uniform ultrathin zirconia gel film using a sol-gel 
      process. Due to the large surface area of the cellulose paper and the strong 
      affinity between zirconia and the cis-diol compounds, the target analytes were 
      selectively extracted from the complex matrix. Thus, the detection sensitivity 
      was greatly improved. Typically, the nanocoating cellulose paper was immersed 
      into the diluted urine for selective extraction of target analytes, then the 
      extracted analytes were subjected to nESI-MS/MS detection. The whole analytical 
      procedure could be completed within 10min. The method was evaluated by the 
      determination of ribonucleosides (adenosine, cytidine, uridine, guanosine) in 
      urine sample. The signal intensities of the ribonuclesides extracted by the 
      nanocoating cellulose paper were greatly enhanced by 136-459-folds compared with 
      the one of the unmodified cellulose paper based microextraction. The limits of 
      detection (LODs) and the limits of quantification (LOQs) of the four 
      ribonucleosides were in the range of 0.0136-1.258mugL(-1) and 0.0454-4.194mugL(-1), 
      respectively. The recoveries of the target nucleosides from spiked human urine 
      were in the range of 75.64-103.49% with the relative standard deviations (RSDs) 
      less than 9.36%. The results demonstrate the potential of the proposed method for 
      rapid and facile determination of endogenous ribonucleosides in urine sample.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Wan, Lingzhong
AU  - Wan L
AD  - Department of Chemistry, School of Chemistry and Materials Science, University of 
      Science and Technology of China (USTC), Hefei 230026, China.
FAU - Zhu, Haijing
AU  - Zhu H
AD  - Department of Chemistry, School of Chemistry and Materials Science, University of 
      Science and Technology of China (USTC), Hefei 230026, China.
FAU - Guan, Yafeng
AU  - Guan Y
AD  - Department of Instrumentation and Analytical Chemistry, Key Laboratory of 
      Separation Science for Analytical Chemistry of CAS, Dalian Institute of Chemical 
      Physics, Chinese Academy of Sciences, Dalian 116023, China.
FAU - Huang, Guangming
AU  - Huang G
AD  - Department of Chemistry, School of Chemistry and Materials Science, University of 
      Science and Technology of China (USTC), Hefei 230026, China. Electronic address: 
      gmhuang@ustc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170328
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Ribonucleosides)
RN  - 9004-34-6 (Cellulose)
RN  - C6V6S92N3C (Zirconium)
RN  - S38N85C5G0 (zirconium oxide)
SB  - IM
MH  - Cellulose/*chemistry
MH  - Humans
MH  - Limit of Detection
MH  - *Paper
MH  - Ribonucleosides/*urine
MH  - Solid Phase Microextraction/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Zirconium/chemistry
OTO - NOTNLM
OT  - Nanocoating cellulose paper based microextraction
OT  - Nanospray mass spectrometry
OT  - Ribonucleosides
OT  - Zirconia
EDAT- 2017/04/17 06:00
MHDA- 2018/08/29 06:00
CRDT- 2017/04/17 06:00
PHST- 2016/11/29 00:00 [received]
PHST- 2017/03/14 00:00 [revised]
PHST- 2017/03/26 00:00 [accepted]
PHST- 2017/04/17 06:00 [entrez]
PHST- 2017/04/17 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
AID - S0039-9140(17)30377-6 [pii]
AID - 10.1016/j.talanta.2017.03.085 [doi]
PST - ppublish
SO  - Talanta. 2017 Jul 1;169:209-215. doi: 10.1016/j.talanta.2017.03.085. Epub 2017 
      Mar 28.