PMID- 28413321
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1226-8453 (Print)
IS  - 2093-4947 (Electronic)
IS  - 1226-8453 (Linking)
VI  - 41
IP  - 2
DP  - 2017 Apr
TI  - The beneficial effect of ginsenosides extracted by pulsed electric field against 
      hydrogen peroxide-induced oxidative stress in HEK-293 cells.
PG  - 169-179
LID - 10.1016/j.jgr.2016.02.007 [doi]
AB  - BACKGROUND: Ginsenosides are the main pharmacological components of Panax ginseng 
      root, which are thought to be primarily responsible for the suppressing effect on 
      oxidative stress. METHODS: 2,2-diphenyl-1-picrylhydrazyl radical scavenging 
      activity and oxygen radical absorption capacity were applied to evaluate the 
      antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) 
      cells were incubated with ginsenosides extracted by pulsed electric field (PEF) 
      and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced 
      by 40muM H(2)O(2). The cell viability and surface morphology of HEK-293 cells were 
      studied using MTS assay and scanning electron microscopy, respectively. 
      Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to 
      measure the level of intracellular reactive oxygen species. The intracellular 
      antioxidant activities of ginsenosides were evaluated by cellular antioxidant 
      activity assay in HepG2 cells. RESULTS: The PEF extracts displayed the higher 
      2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen 
      radical absorption capacity (with an oxygen radical absorption capacity value of 
      14.48 +/- 4.04muM TE per mug/mL). The HEK-293 cell model also suggested that the 
      protective effect of PEF extracts was dose-dependently greater than SCSE 
      extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF 
      extracts are more active (8% higher than SCSE extracts) in reducing intracellular 
      reactive oxygen species accumulation. In addition, scanning electron microscopy 
      images showed that the HEK-293 cells, which were treated with PEF extracts, 
      maintained more intact surface morphology. Cellular antioxidant activity values 
      indicated that ginsenosides extracted by PEF had stronger cellular antioxidant 
      activity than SCSE ginsenosides extracts. CONCLUSION: The present study 
      demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. 
      Furthermore, rather than SCSE, PEF may be more useful as an alternative 
      extraction technique for the extraction of ginsenosides with enhanced antioxidant 
      activity.
FAU - Liu, Di
AU  - Liu D
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Zhang, Ting
AU  - Zhang T
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Chen, Zhifei
AU  - Chen Z
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Ma, Shuang
AU  - Ma S
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Liu, Jiyun
AU  - Liu J
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
FAU - Liu, Jingbo
AU  - Liu J
AD  - Laboratory of Nutrition and Functional Food, College of Food Science and 
      Engineering, Jilin University, Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20160303
PL  - Korea (South)
TA  - J Ginseng Res
JT  - Journal of ginseng research
JID - 100890690
PMC - PMC5386122
OTO - NOTNLM
OT  - Panax ginseng
OT  - antioxidant activities
OT  - ginsenosides
OT  - oxidative stress
OT  - pulsed electric field
EDAT- 2017/04/18 06:00
MHDA- 2017/04/18 06:01
PMCR- 2016/03/03
CRDT- 2017/04/18 06:00
PHST- 2015/09/16 00:00 [received]
PHST- 2016/01/30 00:00 [revised]
PHST- 2016/02/26 00:00 [accepted]
PHST- 2017/04/18 06:00 [entrez]
PHST- 2017/04/18 06:00 [pubmed]
PHST- 2017/04/18 06:01 [medline]
PHST- 2016/03/03 00:00 [pmc-release]
AID - S1226-8453(16)30002-1 [pii]
AID - 10.1016/j.jgr.2016.02.007 [doi]
PST - ppublish
SO  - J Ginseng Res. 2017 Apr;41(2):169-179. doi: 10.1016/j.jgr.2016.02.007. Epub 2016 
      Mar 3.