PMID- 28413484
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 13
IP  - 4
DP  - 2017 Apr
TI  - Inhibitor kappaBalpha protein therapy alleviates severe pneumonia through inhibition of 
      nuclear factor kappaB.
PG  - 1398-1402
LID - 10.3892/etm.2017.4130 [doi]
AB  - To investigate the effect of inhibitor kappaBalpha (IkappaBalpha) on severe pneumonia and explain 
      the mechanisms of nuclear factor kappaB (NF-kappaB), the activation of NF-kappaB was induced 
      in Sprague-Dawley (SD) rats infected with Klebsiella pneumoniae (K. pneumoniae). 
      The rats were then treated with differing concentrations of IkappaBalpha protein. A 
      histological analysis was performed to compare the lung structure prior to and 
      following treatment, and an immunohistochemistry assay was used to detect NF-kappaB 
      activity. In addition, the expression of certain inflammatory factors was 
      detected using a protein chip assay. The severe pneumonia rat model was 
      successfully produced and in model rats, NF-kappaB was activated by K. pneumoniae. 
      Following treatment with IkappaBalpha, the activity of NF-kappaB was inhibited and pneumonia 
      symptoms in model rats were alleviated. Furthermore, the expression of a number 
      of inflammatory factors including tumor necrosis factor alpha (TNF-alpha), interleukin 6 
      (IL-6), interferon gamma (IFN-gamma) and monocyte chemoattractant protein-1 (MCP-1) were 
      also inhibited. The current study demonstrates that NF-kappaB inhibition with IkappaBalpha 
      protein therapy prevents the development of pneumonia in a K. pneumoniae rat 
      model. The therapeutic effect is indicated by the responses of proinflammatory 
      factors, including TNF-alpha, IL-6, IFN-gamma and MCP-1.
FAU - Xu, Haizhou
AU  - Xu H
AD  - Department of Emergency, Changhai Hospital, Second Military Medical University, 
      Shanghai 200433, P.R. China.
FAU - Mei, Bing
AU  - Mei B
AD  - Department of Emergency, Changhai Hospital, Second Military Medical University, 
      Shanghai 200433, P.R. China.
FAU - Wang, Meitang
AU  - Wang M
AD  - Department of Emergency, Changhai Hospital, Second Military Medical University, 
      Shanghai 200433, P.R. China.
FAU - Xu, Shuogui
AU  - Xu S
AD  - Department of Emergency, Changhai Hospital, Second Military Medical University, 
      Shanghai 200433, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170216
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5377337
OTO - NOTNLM
OT  - Klebsiella pneumoniae
OT  - inhibitor kappaBalpha
OT  - nuclear factor kappaB
OT  - protein therapy
EDAT- 2017/04/18 06:00
MHDA- 2017/04/18 06:01
PMCR- 2017/02/16
CRDT- 2017/04/18 06:00
PHST- 2015/09/16 00:00 [received]
PHST- 2016/11/09 00:00 [accepted]
PHST- 2017/04/18 06:00 [entrez]
PHST- 2017/04/18 06:00 [pubmed]
PHST- 2017/04/18 06:01 [medline]
PHST- 2017/02/16 00:00 [pmc-release]
AID - ETM-0-0-4130 [pii]
AID - 10.3892/etm.2017.4130 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Apr;13(4):1398-1402. doi: 10.3892/etm.2017.4130. Epub 2017 Feb 
      16.