PMID- 28415497
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20181202
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 75
DP  - 2017 Jun 1
TI  - Modeling and optimization of gelatin-chitosan micro-carriers preparation for soft 
      tissue engineering: Using Response Surface Methodology.
PG  - 545-553
LID - S0928-4931(17)30702-6 [pii]
LID - 10.1016/j.msec.2017.02.108 [doi]
AB  - Electrospray ionization is a wide spread technique for producing polymeric 
      microcarriers (MCs) by applying electrostatic force and ionic cross-linker, 
      simultaneously. In this study, fabrication process of gelatin-chitosan MCs and 
      its optimization using the Response Surface Methodology (RSM) is reported. 
      Gelatin/chitosan (G/C) blend ratio, applied voltage and feeding flow rate, their 
      individual and interaction effects on the diameter and mechanical strength of the 
      MCs were investigated. The obtained models for diameter and mechanical strength 
      of MCs have a quadratic relationship with G/C blend ratio, applied voltage and 
      feeding flow rate. Using the desirability curve, optimized G/C blend ratios that 
      are introduced, include the desirable quantities for MCs diameter and mechanical 
      strength. MCs of the same desirable diameter (350mum) and different G/C blend 
      ratio (1, 2, and 3) were fabricated and their elasticity was investigated via 
      Atomic Force Microscopy (AFM). The biocompatibility of the MCs was evaluated 
      using MTT assay. The results showed that human Umbilical Cord Mesenchymal Stem 
      Cells (hUCMSCs) could attach and proliferate on fabricated MCs during 7days of 
      culturing especially on those prepared with G/C blend ratios of 1 and 2. Such 
      gelatin-chitosan MCs may be considered as a promising candidate for injectable 
      tissue engineering scaffolds, supporting attachment and proliferation of hUCMSCs.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Radaei, Payam
AU  - Radaei P
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran 11365-8639, Iran.
FAU - Mashayekhan, Shohreh
AU  - Mashayekhan S
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran 11365-8639, Iran. Electronic address: mashayekhan@sharif.edu.
FAU - Vakilian, Saeid
AU  - Vakilian S
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran 11365-8639, Iran; Stem Cell Technology Research Center, Tehran 
      1997775555, Iran.
LA  - eng
PT  - Journal Article
DEP - 20170224
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 9000-70-8 (Gelatin)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Chitosan/*chemistry
MH  - Gelatin/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Chitosan
OT  - Gelatin
OT  - Human umbilical cord mesenchymal stem cells
OT  - Micro-carrier
OT  - Response surface methodology
OT  - Tissue engineering
EDAT- 2017/04/19 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/04/19 06:00
PHST- 2016/07/09 00:00 [received]
PHST- 2016/12/05 00:00 [revised]
PHST- 2017/02/21 00:00 [accepted]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
AID - S0928-4931(17)30702-6 [pii]
AID - 10.1016/j.msec.2017.02.108 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:545-553. doi: 
      10.1016/j.msec.2017.02.108. Epub 2017 Feb 24.