PMID- 28415697
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR  - 20181113
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 17
DP  - 2017 Apr 25
TI  - Down-regulation of interleukin 7 receptor (IL-7R) contributes to central nervous 
      system demyelination.
PG  - 28395-28407
LID - 10.18632/oncotarget.16081 [doi]
AB  - Interleukin 7 receptor (IL-7R) has been associated with the pathogenesis of 
      multiple sclerosis (MS), though the mechanisms are not clear. Because myelin 
      expression is highly conserved between zebrafish and mammals, zebrafish have 
      become an ideal model for studying demyelination. We used a transgenic (Tg; 
      mbp:nfsB-egfp) zebrafish line in which oligodendrocytes expressed green 
      fluorescent protein (GFP) from the larval stage to adulthood. Exposing adult 
      transgenic zebrafish to metronidazole induced demyelination that resembled the 
      morphological changes associated with the early stages of MS. The 
      metronidazole-induced demyelination was confirmed by magnetic resonance imaging 
      (MRI) for the first time. Microarray analysis revealed down-regulation of IL-7R 
      during demyelination. Targeted knockdown of IL-7R demonstrated that IL-7R is 
      essential for myelination in embryonic and larval zebrafish. Moreover, IL-7R 
      down-regulation induced signaling via the JAK/STAT pathway leading to apoptosis 
      in oligodendrocytes. These findings contribute to our understanding of the role 
      of IL-7R in demyelination, and provide a rationale for the development of 
      IL-7R-based therapies for MS and other demyelinating diseases.
FAU - Lei, Xudan
AU  - Lei X
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Cai, Shijiao
AU  - Cai S
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Chen, Yang
AU  - Chen Y
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Cui, Jianlin
AU  - Cui J
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Wang, Yajie
AU  - Wang Y
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Li, Zongjin
AU  - Li Z
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
FAU - Li, Yuhao
AU  - Li Y
AD  - Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai 
      University School of Medicine, Tianjin 300071, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (STAT Transcription Factors)
RN  - EC 2.7.10.2 (Janus Kinases)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Central Nervous System Diseases/diagnostic imaging/*genetics/metabolism/pathology
MH  - Demyelinating Diseases/diagnostic imaging/*genetics/metabolism/pathology
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Genes, bcl-2
MH  - Janus Kinases/metabolism
MH  - Receptors, Interleukin-7/*genetics/metabolism
MH  - STAT Transcription Factors/metabolism
MH  - Signal Transduction
MH  - Spinal Cord/metabolism/pathology
MH  - Zebrafish
PMC - PMC5438658
OTO - NOTNLM
OT  - demyelination
OT  - interleukin 7 receptor (IL-7R)
OT  - myelin basic protein
OT  - myelination
OT  - zebrafish
COIS- CONFLICTS OF INTEREST The authors declare no competing financial interests.
EDAT- 2017/04/19 06:00
MHDA- 2018/03/06 06:00
PMCR- 2017/04/25
CRDT- 2017/04/19 06:00
PHST- 2016/11/08 00:00 [received]
PHST- 2017/02/27 00:00 [accepted]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/04/25 00:00 [pmc-release]
AID - 16081 [pii]
AID - 10.18632/oncotarget.16081 [doi]
PST - ppublish
SO  - Oncotarget. 2017 Apr 25;8(17):28395-28407. doi: 10.18632/oncotarget.16081.