PMID- 28415785
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR  - 20211204
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 18
DP  - 2017 May 2
TI  - The role of nitric oxide pathway in arginine transport and growth of IPEC-1 
      cells.
PG  - 29976-29983
LID - 10.18632/oncotarget.16267 [doi]
AB  - L-Arginine itself and its metabolite-nitric oxide play great roles in intestinal 
      physiology. However, the molecular mechanism underlying nitric oxide pathway 
      regulating L-Arginine transport and cell growth is not yet fully understood. We 
      report that inhibition of nitric oxide synthase (NOS) significantly induced cell 
      apoptosis (p < 0.05), and promoted the rate of Arginine uptake and the 
      expressions of protein for CAT-2 and y+LAT-1 (p < 0.05), while reduced protein 
      expression of CAT-1. And NOS inhibition markedly decreased the activation of 
      mammalian target of rapamycin (mTOR) and PI3K-Akt pathways by Arginine in the 
      IPEC-1 cells (p < 0.05). Taken together, these data suggest that inhibition of NO 
      pathway by L-NAME induces a negative feedback increasing of Arginine uptake and 
      CAT-2 and y+LAT-1 protein expression, but promotes cell apoptosis which involved 
      inhibiting the activation of mTOR and PI3K-Akt pathways.
FAU - Xiao, Hao
AU  - Xiao H
AD  - National Engineering Laboratory for Pollution Control and Waste Utilization in 
      Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in 
      Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of 
      Sciences, Changsha 410125, Hunan, China.
AD  - University of the Chinese Academy of Sciences, Beijing, China.
FAU - Zeng, Liming
AU  - Zeng L
AD  - National Engineering Laboratory for Pollution Control and Waste Utilization in 
      Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in 
      Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of 
      Sciences, Changsha 410125, Hunan, China.
AD  - Science College of Jiangxi Agricultural University, Nanchang, Jiangxi, China.
FAU - Shao, Fangyuan
AU  - Shao F
AD  - Faculty of Health Sciences, University of Macau, Macau SAR, China.
FAU - Huang, Bo
AU  - Huang B
AD  - National Engineering Laboratory for Pollution Control and Waste Utilization in 
      Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in 
      Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of 
      Sciences, Changsha 410125, Hunan, China.
AD  - University of the Chinese Academy of Sciences, Beijing, China.
FAU - Wu, Miaomiao
AU  - Wu M
AD  - Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Tan, Bie
AU  - Tan B
AD  - National Engineering Laboratory for Pollution Control and Waste Utilization in 
      Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in 
      Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of 
      Sciences, Changsha 410125, Hunan, China.
FAU - Yin, Yulong
AU  - Yin Y
AD  - National Engineering Laboratory for Pollution Control and Waste Utilization in 
      Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in 
      Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of 
      Sciences, Changsha 410125, Hunan, China.
AD  - Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal 
      University, Changsha, Hunan, China.
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Arginine/*metabolism
MH  - Biological Transport
MH  - Cells, Cultured
MH  - Epithelial Cells/*metabolism
MH  - *Metabolic Networks and Pathways/drug effects
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Signal Transduction/drug effects
MH  - Swine
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC5444718
OTO - NOTNLM
OT  - arginine
OT  - intestinal porcine epithelial cells (IPEC)
OT  - mTOR
OT  - nitric oxide synthase
COIS- CONFLICTS OF INTEREST None.
EDAT- 2017/04/19 06:00
MHDA- 2018/03/06 06:00
PMCR- 2017/05/02
CRDT- 2017/04/19 06:00
PHST- 2016/12/12 00:00 [received]
PHST- 2017/03/08 00:00 [accepted]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/05/02 00:00 [pmc-release]
AID - 16267 [pii]
AID - 10.18632/oncotarget.16267 [doi]
PST - ppublish
SO  - Oncotarget. 2017 May 2;8(18):29976-29983. doi: 10.18632/oncotarget.16267.