PMID- 28417259
OWN - NLM
STAT- MEDLINE
DCOM- 20180816
LR  - 20200424
IS  - 1437-1596 (Electronic)
IS  - 0937-9827 (Linking)
VI  - 132
IP  - 1
DP  - 2018 Jan
TI  - Comparison between magnetic bead and qPCR library normalisation methods for 
      forensic MPS genotyping.
PG  - 125-132
LID - 10.1007/s00414-017-1591-9 [doi]
AB  - Massively parallel sequencing (MPS) is fast approaching operational use in 
      forensic science, with the capability to analyse hundreds of DNA identity and DNA 
      intelligence markers in multiple samples simultaneously. The ForenSeq DNA 
      Signature Kit on MiSeq FGx (Illumina) workflow can provide profiles for 
      autosomal short tandem repeats (STRs), X chromosome and Y chromosome STRs, 
      identity single nucleotide polymorphisms (SNPs), biogeographical ancestry SNPs 
      and phenotype (eye and hair colour) SNPs from a sample. The library preparation 
      procedure involves a series of steps including target amplification, library 
      purification and library normalisation. This study highlights the comparison 
      between the manufacturer recommended magnetic bead normalisation and quantitative 
      polymerase chain reaction (qPCR) methods. Furthermore, two qPCR chemistries, 
      KAPA(R) (KAPA Biosystems) and NEBNext(R) (New England Bio Inc.), have also been 
      compared. The qPCR outperformed the bead normalisation method, while the NEBNext(R) 
      kit obtained higher genotype concordance than KAPA(R). The study also established 
      an MPS workflow that can be utilised in any operational forensic laboratory.
FAU - Mehta, Bhavik
AU  - Mehta B
AD  - National Centre for Forensic Studies, Faculty of Education, Science, Technology 
      and Mathematics (ESTeM), University of Canberra, Canberra, Bruce, ACT 2617, 
      Australia. bhavik.mehta@hotmail.com.
AD  - Specialist Operations - Forensics, Australian Federal Police, Tambreet Street, 
      Majura, ACT 2609, Australia. bhavik.mehta@hotmail.com.
FAU - Venables, Samantha
AU  - Venables S
AD  - National Centre for Forensic Studies, Faculty of Education, Science, Technology 
      and Mathematics (ESTeM), University of Canberra, Canberra, Bruce, ACT 2617, 
      Australia.
AD  - Specialist Operations - Forensics, Australian Federal Police, Tambreet Street, 
      Majura, ACT 2609, Australia.
FAU - Roffey, Paul
AU  - Roffey P
AD  - National Centre for Forensic Studies, Faculty of Education, Science, Technology 
      and Mathematics (ESTeM), University of Canberra, Canberra, Bruce, ACT 2617, 
      Australia.
AD  - Specialist Operations - Forensics, Australian Federal Police, Tambreet Street, 
      Majura, ACT 2609, Australia.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170418
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
SB  - IM
MH  - *DNA Fingerprinting
MH  - Genotype
MH  - Genotyping Techniques/instrumentation/*methods
MH  - *High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - *Magnets
MH  - *Polymerase Chain Reaction
OTO - NOTNLM
OT  - Forensic DNA profiling
OT  - Illumina MiSeq FGx
OT  - Library normalisation
OT  - Massively parallel sequencing (MPS)
OT  - Next generation sequencing (NGS)
OT  - Quantitative polymerase chain reaction (qPCR)
EDAT- 2017/04/19 06:00
MHDA- 2018/08/17 06:00
CRDT- 2017/04/19 06:00
PHST- 2016/12/06 00:00 [received]
PHST- 2017/04/06 00:00 [accepted]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2018/08/17 06:00 [medline]
PHST- 2017/04/19 06:00 [entrez]
AID - 10.1007/s00414-017-1591-9 [pii]
AID - 10.1007/s00414-017-1591-9 [doi]
PST - ppublish
SO  - Int J Legal Med. 2018 Jan;132(1):125-132. doi: 10.1007/s00414-017-1591-9. Epub 
      2017 Apr 18.