PMID- 28418537
OWN - NLM
STAT- MEDLINE
DCOM- 20180713
LR  - 20190930
IS  - 1560-2281 (Electronic)
IS  - 1083-3668 (Linking)
VI  - 22
IP  - 9
DP  - 2017 Sep 1
TI  - Assessment of decellularization of heart bioimplants using a Raman spectroscopy 
      method.
PG  - 91511
LID - 10.1117/1.JBO.22.9.091511 [doi]
AB  - We report the results of experimental studies on cardiac implants using a Raman 
      spectroscopy method (RS). Raman spectra characteristics of leaves and walls of 
      cardiac implants were obtained; the implants were manufactured by protocols of 
      detergent-enzymatic technique (DET) and biological, detergent-free (BIO) 
      decellularization, using detergents (group DET) or a detergent-free, 
      nonproteolytic, actin-disassembling regimen (BIO). There were input optical 
      coefficients that allowed us to carry out evaluation of the protocols of DET and 
      BIO decellularization on the basis of the concentrations of glycosaminoglycans, 
      proteins, amides, and DNA. It was shown that during DET and BIO 
      decellularization, composition aberrations of proteins and lipids do not occur 
      and the integrity of the collagenous structures is preserved. It was found that 
      during the DET decellularization, preservation of glycosaminoglycans is better 
      than during BIO decellularization.
FAU - Timchenko, Elena V
AU  - Timchenko EV
AD  - Samara National Research University, Samara, Russia.
FAU - Timchenko, Pavel E
AU  - Timchenko PE
AD  - Samara National Research University, Samara, Russia.
FAU - Lichtenberg, Artur
AU  - Lichtenberg A
AD  - Heinrich Heine University, Clinic of Cardiovascular Surgery, Dusseldorf, Germany.
FAU - Assmann, Alexander
AU  - Assmann A
AD  - Heinrich Heine University, Clinic of Cardiovascular Surgery, Dusseldorf, Germany.
FAU - Aubin, Hug
AU  - Aubin H
AD  - Heinrich Heine University, Clinic of Cardiovascular Surgery, Dusseldorf, Germany.
FAU - Akhyari, Payam
AU  - Akhyari P
AD  - Heinrich Heine University, Clinic of Cardiovascular Surgery, Dusseldorf, Germany.
FAU - Volova, Larisa T
AU  - Volova LT
AD  - Experimental Medicine and Biotechnologies Institute of the Samara State Medical 
      University, Samara, Russia.
FAU - Pershutkina, Svetlana V
AU  - Pershutkina SV
AD  - Samara National Research University, Samara, Russia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (Detergents)
SB  - IM
MH  - Animals
MH  - Detergents/metabolism
MH  - *Heart
MH  - *Prostheses and Implants
MH  - *Spectrum Analysis, Raman
EDAT- 2017/04/19 06:00
MHDA- 2018/07/14 06:00
CRDT- 2017/04/19 06:00
PHST- 2016/12/03 00:00 [received]
PHST- 2017/03/22 00:00 [accepted]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2018/07/14 06:00 [medline]
AID - 2621907 [pii]
AID - 10.1117/1.JBO.22.9.091511 [doi]
PST - ppublish
SO  - J Biomed Opt. 2017 Sep 1;22(9):91511. doi: 10.1117/1.JBO.22.9.091511.