PMID- 28419176
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20190208
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - MICA diversity and linkage disequilibrium with HLA-B alleles in renal-transplant 
      candidates in southern Brazil.
PG  - e0176072
LID - 10.1371/journal.pone.0176072 [doi]
LID - e0176072
AB  - The major histocompatibility complex (MHC) class I chain-related gene A (MICA) is 
      located centromerically to the human leukocyte antigen (HLA)-B. The short 
      distance between these loci in the MHC indicates the presence of linkage 
      disequilibrium (LD). Similarly to the HLA, the MICA is highly polymorphic, and 
      this polymorphism has not been well documented in different populations. In this 
      study, we estimated the allelic frequencies of MICA and the linkage 
      disequilibrium with HLA-B alleles in 346 renal-transplant candidates in southern 
      Brazil. MICA and HLA were typed using the polymerase chain 
      reaction-sequence-specific primer method (PCR-SSO), combined with the Luminex 
      technology. A total of 19 MICA allele groups were identified. The most frequent 
      allele groups were MICA*008 (21.6%), MICA*002 (17.0%) and MICA*004 (14.8%). The 
      most common haplotypes were MICA*009-B*51 (7.8%), MICA*004-B*44 (6.06%) and 
      MICA*002-B*35 (5.63%). As expected from the proximity of the MICA and HLA-B loci, 
      most haplotypes showed strong LD. Renal patients and healthy subjects in the same 
      region of Brazil showed statistically significant differences in their MICA 
      polymorphisms. The MICA*027 allele group was more frequent in renal patients (Pc 
      = 0.018, OR: 3.421, 95% CI: 1.516-7.722), while the MICA*019 allele group was 
      more frequent in healthy subjects (Pc = 0.001, OR: 0.027, 95% CI: 0.002-0.469). 
      This study provided information on the distribution of MICA polymorphisms and 
      linkage disequilibrium with HLA-B alleles in Brazilian renal-transplant 
      candidates. This information should help to determine the mechanisms of 
      susceptibility to different diseases in patients with chronic kidney disease, and 
      to elucidate the mechanisms involved in allograft rejection associated with MICA 
      polymorphisms in a Brazilian population.
FAU - Yamakawa, Roger Haruki
AU  - Yamakawa RH
AD  - Department of Basic Health Science, Universidade Estadual de Maringa - Maringa, 
      Parana, Brazil.
FAU - Saito, Patricia Keiko
AU  - Saito PK
AD  - Department of Basic Health Science, Universidade Estadual de Maringa - Maringa, 
      Parana, Brazil.
FAU - Gelmini, Georgia Fernanda
AU  - Gelmini GF
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Department of Genetics, 
      Universidade Federal do Parana - Curitiba, Parana, Brazil.
FAU - da Silva, Jose Samuel
AU  - da Silva JS
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Department of Genetics, 
      Universidade Federal do Parana - Curitiba, Parana, Brazil.
FAU - Bicalho, Maria da Graca
AU  - Bicalho MDG
AD  - Laboratorio de Imunogenetica e Histocompatibilidade, Department of Genetics, 
      Universidade Federal do Parana - Curitiba, Parana, Brazil.
FAU - Borelli, Sueli Donizete
AU  - Borelli SD
AD  - Department of Basic Health Science, Universidade Estadual de Maringa - Maringa, 
      Parana, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170418
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (MHC class I-related chain A)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Brazil
MH  - Female
MH  - HLA-B Antigens/*genetics
MH  - Haplotypes
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - *Kidney Transplantation
MH  - *Linkage Disequilibrium
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Renal Insufficiency, Chronic/*genetics/therapy
PMC - PMC5395226
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/04/19 06:00
MHDA- 2017/05/06 06:00
PMCR- 2017/04/18
CRDT- 2017/04/19 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/04/05 00:00 [accepted]
PHST- 2017/04/19 06:00 [entrez]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2017/04/18 00:00 [pmc-release]
AID - PONE-D-16-46418 [pii]
AID - 10.1371/journal.pone.0176072 [doi]
PST - epublish
SO  - PLoS One. 2017 Apr 18;12(4):e0176072. doi: 10.1371/journal.pone.0176072. 
      eCollection 2017.