PMID- 28419472
OWN - NLM
STAT- MEDLINE
DCOM- 20170914
LR  - 20180130
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 141
IP  - 2
DP  - 2017 Jul 15
TI  - Hepatic expression of oncogenes Bmi1 and Dkk1 is up-regulated in hepatitis B 
      virus surface antigen-transgenic mice and can be induced by treatment with HBV 
      particles or lipopolysaccharides in vitro.
PG  - 354-363
LID - 10.1002/ijc.30742 [doi]
AB  - Previous studies have shown that hepatocellular carcinoma (HCC) develops more 
      frequently in hepatitis B virus surface antigen (HBsAg)-transgenic mice (Alb/HBs) 
      than in wild-type (WT) mice. However, the mechanism of this HCC model has not 
      been well documented. Toll-like receptor 4 (Tlr4) signaling probably links innate 
      immunity and HCC progression. This study was designed to investigate the role of 
      innate immunity in hepatocarcinogenesis in Alb/HBs mice. Immunohistochemical 
      analysis of liver specimens from Alb/HBs mice (16 per group) showed that the 
      oncogenes Bmi1 (16/16, 100%) and Dkk1 (13/16, 81.25%) were highly expressed in 
      Alb/HBs mice, whereas the other oncogenes evaluated were expressed in smaller 
      percentages of mice (Afp, 9/16, 56.2%; Ctnnb1, 5/16, 31.3%; Epcam, 0/16; 0%). 
      Comparable results were obtained by quantitative PCR analysis. Hepatic gene 
      expression of Tlr2, Tlr4, Il6 and Tnf was additionally elevated in Alb/HBs mice. 
      Stimulation of primary murine hepatocytes with cell culture-derived HBV particles 
      or LPS increased the expression of oncogenes (Bmi1, Dkk1) and inflammatory 
      factors (Tnf, Il6, Tlr4). Proliferation and colony formation of hepatoma cells 
      were enhanced by treatment with HBV and LPS and were impaired by the suppression 
      of Bmi1 and Dkk1 by small interfering RNAs. Substantial induction of BMI1 and 
      DKK1 was found in liver biopsy samples from patients with HBV-related HCC but not 
      in HCC samples without HBV infection background. These findings suggest that 
      innate immunity may link inflammation and tumor progression during chronic HBV 
      infection, involving the oncogenes BMI1 and DKK1.
CI  - (c) 2017 UICC.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Gastroenterology and Hepatology, University Hospital Essen, 
      University Duisburg-Essen, Germany.
AD  - Department of Biliary-Pancreatic Surgery, Sun Yat-sen Memorial Hospital, Sun 
      Yat-sen University, Guangzhou, China.
FAU - Real, Catherine I
AU  - Real CI
AD  - Department of Gastroenterology and Hepatology, University Hospital Essen, 
      University Duisburg-Essen, Germany.
FAU - Liu, Chao
AU  - Liu C
AD  - Department of Biliary-Pancreatic Surgery, Sun Yat-sen Memorial Hospital, Sun 
      Yat-sen University, Guangzhou, China.
FAU - Baba, Hideo A
AU  - Baba HA
AD  - Department of Pathology and Neuropathology, University Hospital Essen, University 
      Duisburg-Essen, Germany.
FAU - Gerken, Guido
AU  - Gerken G
AD  - Department of Gastroenterology and Hepatology, University Hospital Essen, 
      University Duisburg-Essen, Germany.
FAU - Lu, Mengji
AU  - Lu M
AD  - Institute of Virology, University Hospital Essen, University Duisburg-Essen, 
      Germany.
FAU - Broering, Ruth
AU  - Broering R
AUID- ORCID: 0000-0002-7125-3246
AD  - Department of Gastroenterology and Hepatology, University Hospital Essen, 
      University Duisburg-Essen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170508
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (BMI1 protein, human)
RN  - 0 (DKK1 protein, human)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - EC 2.3.2.27 (Polycomb Repressive Complex 1)
SB  - IM
MH  - Animals
MH  - Carcinoma, Hepatocellular/genetics/immunology/metabolism/*virology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Gene Expression Regulation, Neoplastic
MH  - Hep G2 Cells
MH  - Hepatitis B Surface Antigens/*genetics/metabolism
MH  - Hepatocytes/cytology/drug effects/metabolism/virology
MH  - Humans
MH  - Immunity, Innate
MH  - Intercellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Liver Neoplasms/genetics/immunology/metabolism/*virology
MH  - Mice
MH  - Mice, Transgenic
MH  - Polycomb Repressive Complex 1/genetics/*metabolism
MH  - *Up-Regulation
MH  - Virion/pathogenicity
OTO - NOTNLM
OT  - HBsAg
OT  - HCC
OT  - LPS
OT  - hepatocellular carcinoma
OT  - innate immunity
EDAT- 2017/04/19 06:00
MHDA- 2017/09/15 06:00
CRDT- 2017/04/19 06:00
PHST- 2016/07/12 00:00 [received]
PHST- 2017/04/03 00:00 [accepted]
PHST- 2017/04/19 06:00 [pubmed]
PHST- 2017/09/15 06:00 [medline]
PHST- 2017/04/19 06:00 [entrez]
AID - 10.1002/ijc.30742 [doi]
PST - ppublish
SO  - Int J Cancer. 2017 Jul 15;141(2):354-363. doi: 10.1002/ijc.30742. Epub 2017 May 
      8.