PMID- 28420144
OWN - NLM
STAT- MEDLINE
DCOM- 20170629
LR  - 20181202
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Apr 15
TI  - Retinoids Regulate Adipogenesis Involving the TGFbeta/SMAD and Wnt/beta-Catenin 
      Pathways in Human Bone Marrow Mesenchymal Stem Cells.
LID - 10.3390/ijms18040842 [doi]
LID - 842
AB  - Retinoids may regulate cell differentiation as ligands of retinoic acid receptors 
      (RARs) and/or retinoid X receptors (RXRs). We showed that RAR agonists promoted 
      adipogenesis by upregulating the expression of CCAAT/enhancer-binding protein beta 
      (C/EBPbeta) in the early stages, but blocked adipogenesis at a later stage in human 
      bone marrow mesenchymal stem cells (hBMSCs). RXR agonists promoted adipogenesis 
      at all time points in hBMSCs. The effect of RAR agonists was mediated mainly by 
      the RARbeta subtype. RAR agonists, in contrast to RXR agonists, significantly 
      promoted the expression of RARbeta. Knockdown of the RARbeta gene via small hairpin RNA 
      (shRNA) attenuated the inhibition of RAR agonists toward adipogenesis. 
      Furthermore, we found that RAR agonists upregulated the transforming growth 
      factor beta (TGFbeta)/SMAD pathway and Wnt/beta-catenin pathway on adipogenesis in hBMSCs, 
      and the stimulating effects were noticeably decreased with the RARbeta gene 
      knockdown. Both RAR agonists and RXR agonists inhibited adipogenesis and blocked 
      the promoter activity of C/EBPbeta and peroxisome proliferator-activated receptor gamma 
      (PPARgamma) in SW872 cell. These results indicated the RAR agonists perform dual 
      roles in adipogenesis in hBMSCs, and the TGFbeta/SMAD pathway and Wnt/beta-catenin 
      pathway may involve the inhibitory effect of RAR agonists. RARbeta is the main 
      receptor subtype mediating the effect. The roles of RXR agonists in adipogenesis 
      exhibited cell type-specific differences, and may be based on the integration of 
      signals from different RXR dimers.
FAU - Cao, Jun
AU  - Cao J
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Wuhan University, Wuhan 430071, China. Juncao@whu.edu.cn.
FAU - Ma, Yuhong
AU  - Ma Y
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Wuhan University, Wuhan 430071, China. yuhongma@whu.edu.cn.
FAU - Yao, Weiqi
AU  - Yao W
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Wuhan University, Wuhan 430071, China. weiqiyao@whu.edu.cn.
FAU - Zhang, Xiaoye
AU  - Zhang X
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Wuhan University, Wuhan 430071, China. xiaoyezhang@whu.edu.cn.
FAU - Wu, Dongcheng
AU  - Wu D
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Wuhan University, Wuhan 430071, China. bcdcwu@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20170415
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (PPAR gamma)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Retinoids)
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (retinoic acid receptor beta)
SB  - IM
MH  - Adipogenesis/*drug effects/genetics
MH  - CCAAT-Enhancer-Binding Protein-beta/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - PPAR gamma/metabolism
MH  - Receptors, Retinoic Acid/metabolism
MH  - Retinoid X Receptors/metabolism
MH  - Retinoids/*pharmacology
MH  - Smad Proteins/*metabolism
MH  - Transcriptional Activation/drug effects
MH  - Transforming Growth Factor beta/*metabolism
MH  - *Wnt Signaling Pathway
PMC - PMC5412426
OTO - NOTNLM
OT  - TGFbeta/SMAD pathway
OT  - Wnt/beta-catenin pathway
OT  - adipogenesis
OT  - human bone marrow mesenchymal stem cell
OT  - retinoic acid receptor beta (RARbeta)
OT  - retinoid
COIS- The authors declare no conflict of interest.
EDAT- 2017/04/20 06:00
MHDA- 2017/07/01 06:00
PMCR- 2017/04/01
CRDT- 2017/04/20 06:00
PHST- 2017/03/06 00:00 [received]
PHST- 2017/03/31 00:00 [revised]
PHST- 2017/04/06 00:00 [accepted]
PHST- 2017/04/20 06:00 [entrez]
PHST- 2017/04/20 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijms18040842 [pii]
AID - ijms-18-00842 [pii]
AID - 10.3390/ijms18040842 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Apr 15;18(4):842. doi: 10.3390/ijms18040842.