PMID- 28420943
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20181202
IS  - 1918-1523 (Electronic)
IS  - 1203-6765 (Print)
IS  - 1203-6765 (Linking)
VI  - 2017
DP  - 2017
TI  - Neural Mobilization Treatment Decreases Glial Cells and Brain-Derived 
      Neurotrophic Factor Expression in the Central Nervous System in Rats with 
      Neuropathic Pain Induced by CCI in Rats.
PG  - 7429761
LID - 10.1155/2017/7429761 [doi]
LID - 7429761
AB  - Background. Glial cells are implicated in the development of chronic pain and 
      brain-derived neurotropic factor (BDNF) released from activated microglia 
      contributes to the nociceptive transmission. Neural mobilization (NM) technique 
      is a method clinically effective in reducing pain sensitivity. Here we examined 
      the involvement of glial cells and BDNF expression in the thalamus and midbrain 
      after NM treatment in rats with chronic constriction injury (CCI). CCI was 
      induced and rats were subsequently submitted to 10 sessions of NM, every other 
      day, beginning 14 days after CCI. Thalamus and midbrain were analyzed for glial 
      fibrillary acidic protein (GFAP), microglial cell OX-42, and BDNF using 
      Immunohistochemistry and Western blot assays. Results. Thalamus and midbrain of 
      CCI group showed increases in GFAP, OX-42, and BDNF expression compared with 
      control group and, in contrast, showed decreases in GFAP, OX-42, and BDNF after 
      NM when compared with CCI group. The decreased immunoreactivity for GFAP, OX-42, 
      and BDNF in ventral posterolateral nucleus in thalamus and the periaqueductal 
      gray in midbrain was shown by immunohistochemistry. Conclusions. These findings 
      may improve the knowledge about the involvement of astrocytes, microglia, and 
      BDNF in the chronic pain and show that NM treatment, which alleviates neuropathic 
      pain, affects glial cells and BDNF expression.
FAU - Giardini, Aline Carolina
AU  - Giardini AC
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Dos Santos, Fabio Martinez
AU  - Dos Santos FM
AUID- ORCID: 0000-0002-0415-1960
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
AD  - University Nove de Julho, Sao Paulo, SP, Brazil.
FAU - da Silva, Joyce Teixeira
AU  - da Silva JT
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - de Oliveira, Mara Evany
AU  - de Oliveira ME
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Martins, Daniel Oliveira
AU  - Martins DO
AUID- ORCID: 0000-0003-3441-1820
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Chacur, Marucia
AU  - Chacur M
AUID- ORCID: 0000-0002-0172-4110
AD  - Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute 
      of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170322
PL  - United States
TA  - Pain Res Manag
JT  - Pain research & management
JID - 9612504
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cd53 protein, rat)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Tetraspanin 25)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Count
MH  - Central Nervous System/*metabolism/*pathology
MH  - Densitometry
MH  - Disease Models, Animal
MH  - Exercise Therapy/*methods
MH  - *Gene Expression Regulation
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Male
MH  - Neuralgia/pathology/*rehabilitation
MH  - Neuroglia/metabolism/*pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Tetraspanin 25/metabolism
PMC - PMC5380850
EDAT- 2017/04/20 06:00
MHDA- 2017/04/30 06:00
PMCR- 2017/01/01
CRDT- 2017/04/20 06:00
PHST- 2016/09/27 00:00 [received]
PHST- 2017/02/08 00:00 [revised]
PHST- 2017/02/20 00:00 [accepted]
PHST- 2017/04/20 06:00 [entrez]
PHST- 2017/04/20 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.1155/2017/7429761 [doi]
PST - ppublish
SO  - Pain Res Manag. 2017;2017:7429761. doi: 10.1155/2017/7429761. Epub 2017 Mar 22.