PMID- 28422506
OWN - NLM
STAT- MEDLINE
DCOM- 20190712
LR  - 20200930
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 17
IP  - 5
DP  - 2017 May 10
TI  - Extra-Large Pore Mesoporous Silica Nanoparticles for Directing in Vivo M2 
      Macrophage Polarization by Delivering IL-4.
PG  - 2747-2756
LID - 10.1021/acs.nanolett.6b04130 [doi]
AB  - Over the past decade, mesoporous silica nanoparticles (MSNs) smaller than 200 nm 
      with a high colloidal stability have been extensively studied for systemic drug 
      delivery. Although small molecule delivery via MSNs has been successful, the 
      encapsulation of large therapeutic biomolecules, such as proteins or DNA, is 
      limited due to small pore size of the conventional MSNs obtained by 
      soft-templating. Here, we report the synthesis of mesoporous silica nanoparticles 
      with extra-large pores (XL-MSNs) and their application to in vivo cytokine 
      delivery for macrophage polarization. Uniform, size-controllable XL-MSNs with 30 
      nm extra-large pores were synthesized using organic additives and inorganic seed 
      nanoparticles. XL-MSNs showed significantly higher loadings for the model 
      proteins with different molecular weights compared to conventional small pore 
      MSNs. XL-MSNs were used to deliver IL-4, which is an M2-polarizing cytokine and 
      very quickly degraded in vivo, to macrophages and polarize them to 
      anti-inflammatory M2 macrophages in vivo. XL-MSNs induced a low level of reactive 
      oxygen species (ROS) production and no pro-inflammatory cytokines in bone 
      marrow-derived macrophages (BMDMs) and in mice injected intravenously with 
      XL-MSNs. We found that the injected XL-MSNs were targeted to phagocytic myeloid 
      cells, such as neutrophils, monocytes, macrophages, and dendritic cells. Finally, 
      we demonstrated that the injection of IL-4-loaded XL-MSNs successfully triggered 
      M2 macrophage polarization in vivo, suggesting the clinical potential of XL-MSNs 
      for modulating immune systems via targeted delivery of various cytokines.
FAU - Kwon, Dohyeong
AU  - Kwon D
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology , Daejeon 34141, Republic of Korea.
FAU - Cha, Bong Geun
AU  - Cha BG
AD  - School of Chemical Engineering, Sungkyunkwan University , Suwon 16419, Republic 
      of Korea.
FAU - Cho, Yuri
AU  - Cho Y
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology , Daejeon 34141, Republic of Korea.
FAU - Min, Jiyoun
AU  - Min J
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology , Daejeon 34141, Republic of Korea.
FAU - Park, Eun-Byeol
AU  - Park EB
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology , Daejeon 34141, Republic of Korea.
FAU - Kang, Suk-Jo
AU  - Kang SJ
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology , Daejeon 34141, Republic of Korea.
FAU - Kim, Jaeyun
AU  - Kim J
AUID- ORCID: 0000-0002-4687-6732
AD  - School of Chemical Engineering, Sungkyunkwan University , Suwon 16419, Republic 
      of Korea.
AD  - Department of Health Sciences and Technology, Samsung Advanced Institute for 
      Health Science & Technology (SAIHST), Sungkyunkwan University , Suwon 16419, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170424
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Drug Carriers)
RN  - 0 (Reactive Oxygen Species)
RN  - 207137-56-2 (Interleukin-4)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Polarity
MH  - Cell Survival/drug effects
MH  - Drug Carriers/*chemistry
MH  - Drug Liberation
MH  - Humans
MH  - Interleukin-4/administration & dosage/*chemistry
MH  - Macrophages/*drug effects/physiology
MH  - Mice
MH  - Nanoparticles/*chemistry/toxicity
MH  - Porosity
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species/metabolism
MH  - Silicon Dioxide/*chemistry
OTO - NOTNLM
OT  - IL-4
OT  - M2 macrophage polarization
OT  - Mesoporous silica nanoparticle
OT  - cytokine
OT  - large pore
EDAT- 2017/04/20 06:00
MHDA- 2019/07/13 06:00
CRDT- 2017/04/20 06:00
PHST- 2017/04/20 06:00 [pubmed]
PHST- 2019/07/13 06:00 [medline]
PHST- 2017/04/20 06:00 [entrez]
AID - 10.1021/acs.nanolett.6b04130 [doi]
PST - ppublish
SO  - Nano Lett. 2017 May 10;17(5):2747-2756. doi: 10.1021/acs.nanolett.6b04130. Epub 
      2017 Apr 24.