PMID- 28423874
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1011-2367 (Print)
IS  - 1976-5517 (Electronic)
IS  - 1011-2367 (Linking)
VI  - 30
IP  - 12
DP  - 2017 Dec
TI  - The relationship between the variants in the 5'-untranslated regions of equine 
      chorionic gonadotropin genes and serum equine chorionic gonadotropin levels.
PG  - 1679-1683
LID - 10.5713/ajas.17.0133 [doi]
AB  - OBJECTIVE: An experiment was conducted to study the association between the 
      single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of 
      equine chorionic gonadotropin (eCG) genes and the serum eCG levels. METHODS: SNPs 
      in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese 
      indigenous breeds and 3 imported breeds using iPLEX chemistry, and the 
      association between the serum eCG levels of 174 pregnant Da'an mares and their 
      serum eCG levels (determined with ELISA) was analyzed. RESULTS: Four SNPs were 
      identified in the 5'-UTR of the eCGalpha gene, and one of them was unique in the 
      indigenous breeds. There were 2 SNPs detected at the 5' end of the eCGbeta subunit 
      gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C 
      at the 5'-UTR of eCGalpha was associated significantly with eCG levels of 75-day 
      pregnant mare serum (p<0.05) in Da'an mares. Prediction analysis on binding sites 
      of transcription factors showed that the g.39948246T>C mutation causes appearance 
      of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 
      (HFH-2), which is a transcriptional repressor belonging to the forkhead protein 
      family of transcription factors. CONCLUSION: The SNP g.39948246T>C at the 5'-UTR 
      of eCGalpha is associated with eCG levels of 75-day pregnant mare serum (p<0.05).
FAU - Liu, ShuQin
AU  - Liu S
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
FAU - Lian, Song
AU  - Lian S
AD  - College of Animal Science and Technology, China Agricultural University, Beijing 
      100193, China.
FAU - Yang, YunZhou
AU  - Yang Y
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
FAU - Fu, ChunZheng
AU  - Fu C
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
FAU - Ma, HongYing
AU  - Ma H
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
FAU - Xiong, ZhiYao
AU  - Xiong Z
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
FAU - Ling, Yao
AU  - Ling Y
AD  - College of Animal Science and Technology, China Agricultural University, Beijing 
      100193, China.
FAU - Zhao, ChunJiang
AU  - Zhao C
AD  - Equine Center, China Agricultural University, Beijing 100193, China.
AD  - College of Animal Science and Technology, China Agricultural University, Beijing 
      100193, China.
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of 
      Agriculture, 100193, China.
AD  - National Engineering Laboratory for Animal Breeding, Beijing 100193, China.
LA  - eng
PT  - Journal Article
DEP - 20170419
PL  - Korea (South)
TA  - Asian-Australas J Anim Sci
JT  - Asian-Australasian journal of animal sciences
JID - 9884245
PMC - PMC5666169
OTO - NOTNLM
OT  - Equine Chorionic Gonadotropin (eCG)
OT  - Single Nucleotide Polymorphism (SNP)
OT  - Transcription Factor
COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any 
      financial organization regarding the material discussed in the manuscript.
EDAT- 2017/04/21 06:00
MHDA- 2017/04/21 06:01
PMCR- 2017/12/01
CRDT- 2017/04/21 06:00
PHST- 2017/02/15 00:00 [received]
PHST- 2017/03/23 00:00 [revised]
PHST- 2017/03/30 00:00 [accepted]
PHST- 2017/04/21 06:00 [pubmed]
PHST- 2017/04/21 06:01 [medline]
PHST- 2017/04/21 06:00 [entrez]
PHST- 2017/12/01 00:00 [pmc-release]
AID - ajas.17.0133 [pii]
AID - ajas-30-12-1679 [pii]
AID - 10.5713/ajas.17.0133 [doi]
PST - ppublish
SO  - Asian-Australas J Anim Sci. 2017 Dec;30(12):1679-1683. doi: 10.5713/ajas.17.0133. 
      Epub 2017 Apr 19.