PMID- 28424334 OWN - NLM STAT- MEDLINE DCOM- 20170626 LR - 20180427 IS - 1946-6242 (Electronic) IS - 1946-6234 (Linking) VI - 9 IP - 386 DP - 2017 Apr 19 TI - Glycosaminoglycan-based hydrogels capture inflammatory chemokines and rescue defective wound healing in mice. LID - eaai9044 [pii] LID - 10.1126/scitranslmed.aai9044 [doi] AB - Excessive production of inflammatory chemokines can cause chronic inflammation and thus impair cutaneous wound healing. Capturing chemokine signals using wound dressing materials may offer powerful new treatment modalities for chronic wounds. Here, a modular hydrogel based on end-functionalized star-shaped polyethylene glycol (starPEG) and derivatives of the glycosaminoglycan (GAG) heparin was customized for maximal chemokine sequestration. The material is shown to effectively scavenge the inflammatory chemokines MCP-1 (monocyte chemoattractant protein-1), IL-8 (interleukin-8), and MIP-1alpha (macrophage inflammatory protein-1alpha) and MIP-1beta (macrophage inflammatory protein-1beta) in wound fluids from patients suffering from chronic venous leg ulcers and to reduce the migratory activity of human monocytes and polymorphonuclear neutrophils. In an in vivo model of delayed wound healing (db/db mice), starPEG-GAG hydrogels outperformed the standard-of-care product Promogran with respect to reduction of inflammation, as well as increased granulation tissue formation, vascularization, and wound closure. CI - Copyright (c) 2017, American Association for the Advancement of Science. FAU - Lohmann, Nadine AU - Lohmann N AUID- ORCID: 0000-0003-1604-9653 AD - Department of Dermatology, Venerology, and Allergology, Leipzig University, 04103 Leipzig, Germany. AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. FAU - Schirmer, Lucas AU - Schirmer L AUID- ORCID: 0000-0003-1132-808X AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. AD - Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany. FAU - Atallah, Passant AU - Atallah P AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. AD - Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany. FAU - Wandel, Elke AU - Wandel E AUID- ORCID: 0000-0003-0027-5621 AD - Department of Dermatology, Venerology, and Allergology, Leipzig University, 04103 Leipzig, Germany. AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. FAU - Ferrer, Ruben A AU - Ferrer RA AD - Department of Dermatology, Venerology, and Allergology, Leipzig University, 04103 Leipzig, Germany. AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. FAU - Werner, Carsten AU - Werner C AUID- ORCID: 0000-0003-0189-3448 AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. AD - Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany. AD - Technische Universitat Dresden, Center for Regenerative Therapies Dresden, Fetscherstrasse 105, 01307 Dresden, Germany. FAU - Simon, Jan C AU - Simon JC AUID- ORCID: 0000-0003-0073-6061 AD - Department of Dermatology, Venerology, and Allergology, Leipzig University, 04103 Leipzig, Germany. AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. FAU - Franz, Sandra AU - Franz S AUID- ORCID: 0000-0002-3449-2723 AD - Department of Dermatology, Venerology, and Allergology, Leipzig University, 04103 Leipzig, Germany. sandra.franz@medizin.uni-leipzig.de freudenberg@ipfdd.de. AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. FAU - Freudenberg, Uwe AU - Freudenberg U AUID- ORCID: 0000-0002-1359-1095 AD - Collaborative Research Center (SFB-TR67) "Functional Biomaterials for Controlling Healing Processes in Bone and Skin-From Material Science to Clinical Application," Leipzig and Dresden, Germany. sandra.franz@medizin.uni-leipzig.de freudenberg@ipfdd.de. AD - Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Sci Transl Med JT - Science translational medicine JID - 101505086 RN - 0 (Chemokine CCL2) RN - 0 (Chemokines) RN - 0 (Glycosaminoglycans) RN - 0 (Hydrogels) RN - 0 (Interleukin-8) SB - IM MH - Animals MH - Chemokine CCL2/metabolism MH - Chemokines/*metabolism MH - Glycosaminoglycans/*chemistry MH - Hydrogels/*chemistry/pharmacology MH - Interleukin-8/metabolism MH - Leg Ulcer/*metabolism MH - Mice MH - Monocytes/metabolism MH - Neutrophils/metabolism MH - Wound Healing/drug effects EDAT- 2017/04/21 06:00 MHDA- 2017/06/27 06:00 CRDT- 2017/04/21 06:00 PHST- 2016/08/29 00:00 [received] PHST- 2017/01/17 00:00 [revised] PHST- 2017/03/29 00:00 [accepted] PHST- 2017/04/21 06:00 [entrez] PHST- 2017/04/21 06:00 [pubmed] PHST- 2017/06/27 06:00 [medline] AID - 9/386/eaai9044 [pii] AID - 10.1126/scitranslmed.aai9044 [doi] PST - ppublish SO - Sci Transl Med. 2017 Apr 19;9(386):eaai9044. doi: 10.1126/scitranslmed.aai9044.