PMID- 28425952
OWN - NLM
STAT- MEDLINE
DCOM- 20171026
LR  - 20220311
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Apr 20
TI  - Antioxidant and Anti-Senescence Effect of Metformin on Mouse Olfactory 
      Ensheathing Cells (mOECs) May Be Associated with Increased Brain-Derived 
      Neurotrophic Factor Levels-An Ex Vivo Study.
LID - 10.3390/ijms18040872 [doi]
LID - 872
AB  - Metformin, the popular anti-diabetic drug was shown to exert multiple biological 
      effects. The most recent metformin gained attention as an agent that mobilizes 
      endogenous progenitor cells and enhances regenerative potential of organisms, for 
      example by promoting neurogenesis. In the present study, we examined the role of 
      metformin on mouse olfactory ensheathing cells (mOECs) derived from animals 
      receiving metformin for eight weeks at a concentration equal to 2.8 mg/day. The 
      mOECs expanded ex vivo were characterized in terms of their cellular phenotype, 
      morphology, proliferative activity, viability and accumulation of oxidative 
      stress factors. Moreover, we determined the mRNA and protein levels of 
      brain-derived neurotrophic factor (BDNF), distinguishing the secretion of BDNF by 
      mOECs in cultures and circulating serum levels of BDNF. The mOECs used in the 
      experiment were glial fibrillary acidic protein (GFAP) and p75 neurotrophin 
      receptor (p75(NTR)) positive and exhibited both astrocyte-like and non-myelin 
      Schwann cell-like morphologies. Our results revealed that the proliferation of 
      OECs derived from mice treated with metformin was lowered, when compared to 
      control group. Simultaneously, we noted increased cell viability, reduced 
      expression of markers associated with cellular senescence and a decreased amount 
      of reactive oxygen species. We observed increased mRNA expression of BDNF and its 
      down-stream genes. Obtained results indicate that metformin may exert 
      antioxidant, anti-apoptotic and senolytic action on OECs expanded ex vivo.
FAU - Smieszek, Agnieszka
AU  - Smieszek A
AD  - Department of Experimental Biology and Electron Microscope Facility, The Faculty 
      of Biology and Animal Science, Wroclaw University of Environmental and Life 
      Sciences, Norwida 25, 50-375 Wroclaw, Poland. agnieszka.smieszek@upwr.edu.pl.
FAU - Strek, Zuzanna
AU  - Strek Z
AD  - Department of Experimental Biology and Electron Microscope Facility, The Faculty 
      of Biology and Animal Science, Wroclaw University of Environmental and Life 
      Sciences, Norwida 25, 50-375 Wroclaw, Poland. zuzanna.strek@gmail.com.
FAU - Kornicka, Katarzyna
AU  - Kornicka K
AD  - Department of Experimental Biology and Electron Microscope Facility, The Faculty 
      of Biology and Animal Science, Wroclaw University of Environmental and Life 
      Sciences, Norwida 25, 50-375 Wroclaw, Poland. kornicka.katarzyna@gmail.com.
FAU - Grzesiak, Jakub
AU  - Grzesiak J
AD  - Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland. 
      grzesiak.kuba@gmail.com.
FAU - Weiss, Christine
AU  - Weiss C
AD  - PferdePraxis Dr. Med. Vet. Daniel Weiss, Postmatte 14, CH-8807 Freienbach, 
      Switzerland. d.weiss@horsedoc.ch.
FAU - Marycz, Krzysztof
AU  - Marycz K
AD  - Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland. 
      krzysztof.marycz@up.wroc.pl.
LA  - eng
PT  - Journal Article
DEP - 20170420
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Biomarkers
MH  - Brain-Derived Neurotrophic Factor/blood/*metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Metformin/*pharmacology
MH  - Mice
MH  - Mitochondria/drug effects/metabolism
MH  - Neuroglia/cytology/*drug effects/*metabolism
MH  - Olfactory Bulb/*cytology
MH  - Oxidative Stress/drug effects/genetics
MH  - Phenotype
MH  - Signal Transduction/drug effects
PMC - PMC5412453
OTO - NOTNLM
OT  - anti-oxidative
OT  - brain-derived neurotrophic factor
OT  - metformin
OT  - olfactory ensheathing cells
OT  - senolytic
COIS- The authors declare no conflict of interest.
EDAT- 2017/04/21 06:00
MHDA- 2017/10/27 06:00
PMCR- 2017/04/01
CRDT- 2017/04/21 06:00
PHST- 2017/03/15 00:00 [received]
PHST- 2017/04/11 00:00 [revised]
PHST- 2017/04/13 00:00 [accepted]
PHST- 2017/04/21 06:00 [entrez]
PHST- 2017/04/21 06:00 [pubmed]
PHST- 2017/10/27 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijms18040872 [pii]
AID - ijms-18-00872 [pii]
AID - 10.3390/ijms18040872 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Apr 20;18(4):872. doi: 10.3390/ijms18040872.