PMID- 28427248
OWN - NLM
STAT- MEDLINE
DCOM- 20180201
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 40
IP  - 4
DP  - 2017 Apr
TI  - Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact 
      Respiration in Budding Yeast.
PG  - 307-313
LID - 10.14348/molcells.2017.2279 [doi]
AB  - Caloric restriction (CR) has been shown to extend lifespan and prevent cellular 
      senescence in various species ranging from yeast to humans. Many effects of CR 
      may contribute to extend lifespan. Specifically, CR prevents oxidative damage 
      from reactive oxygen species (ROS) by enhancing mitochondrial function. In this 
      study, we characterized 33 single electron transport chain (ETC) gene-deletion 
      strains to identify CR-induced chronological lifespan (CLS) extension mechanisms. 
      Interestingly, defects in 17 of these 33 ETC gene-deleted strains showed loss of 
      both respiratory function and CR-induced CLS extension. On the contrary, the 
      other 16 respiration-capable mutants showed increased CLS upon CR along with 
      increased mitochondrial membrane potential (MMP) and intracellular adenosine 
      triphosphate (ATP) levels, with decreased mitochondrial superoxide generation. We 
      measured the same parameters in the 17 non-respiratory mutants upon CR. CR 
      simultaneously increased MMP and mitochondrial superoxide generation without 
      altering intracellular ATP levels. In conclusion, respiration is essential for 
      CLS extension by CR and is important for balancing MMP, ROS, and ATP levels.
FAU - Kwon, Young-Yon
AU  - Kwon YY
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Lee, Sung-Keun
AU  - Lee SK
AD  - Department of Pharmacology, College of Medicine, Inha University, Incheon 22212, 
      Korea.
FAU - Lee, Cheol-Koo
AU  - Lee CK
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
LA  - eng
PT  - Journal Article
DEP - 20170420
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Electron Transport Chain Complex Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 11062-77-4 (Superoxides)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Cell Respiration/physiology
MH  - Cellular Senescence/*physiology
MH  - Electron Transport Chain Complex Proteins/genetics/*metabolism
MH  - *Energy Metabolism
MH  - Membrane Potential, Mitochondrial/physiology
MH  - Mitochondria/genetics/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Saccharomyces cerevisiae/*metabolism
MH  - Superoxides/metabolism
MH  - Time Factors
PMC - PMC5424277
OTO - NOTNLM
OT  - caloric restriction
OT  - chronological lifespan
OT  - electron transport chain
OT  - mitochondria
OT  - respiration
EDAT- 2017/04/22 06:00
MHDA- 2018/02/02 06:00
PMCR- 2017/04/20
CRDT- 2017/04/22 06:00
PHST- 2016/11/16 00:00 [received]
PHST- 2017/03/26 00:00 [revised]
PHST- 2017/04/07 00:00 [accepted]
PHST- 2017/04/22 06:00 [pubmed]
PHST- 2018/02/02 06:00 [medline]
PHST- 2017/04/22 06:00 [entrez]
PHST- 2017/04/20 00:00 [pmc-release]
AID - S1016-8478(23)00614-3 [pii]
AID - molce-40-4-307 [pii]
AID - 10.14348/molcells.2017.2279 [doi]
PST - ppublish
SO  - Mol Cells. 2017 Apr;40(4):307-313. doi: 10.14348/molcells.2017.2279. Epub 2017 
      Apr 20.