PMID- 28428369 OWN - NLM STAT- MEDLINE DCOM- 20171129 LR - 20231105 IS - 1095-9203 (Electronic) IS - 0036-8075 (Print) IS - 0036-8075 (Linking) VI - 356 IP - 6335 DP - 2017 Apr 21 TI - Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. LID - 10.1126/science.aah4573 [doi] LID - eaah4573 AB - Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C(+) subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease. CI - Copyright (c) 2017, American Association for the Advancement of Science. FAU - Villani, Alexandra-Chloe AU - Villani AC AUID- ORCID: 0000-0001-7461-0408 AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. cvillani@broadinstitute.org m.a.haniffa@newcastle.ac.uk aregev@broadinstitute.org nhacohen@mgh.harvard.edu. AD - Center for Cancer Research, Massachusetts General Hospital, Department of Medicine, Boston, MA, USA. FAU - Satija, Rahul AU - Satija R AUID- ORCID: 0000-0001-9448-8833 AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - New York Genome Center, New York University Center for Genomics and Systems Biology, New York, NY, USA. AD - New York University Center for Genomics and Systems Biology, New York, NY, USA. FAU - Reynolds, Gary AU - Reynolds G AUID- ORCID: 0000-0002-8142-8708 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Sarkizova, Siranush AU - Sarkizova S AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Shekhar, Karthik AU - Shekhar K AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Fletcher, James AU - Fletcher J AUID- ORCID: 0000-0001-9610-1858 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Griesbeck, Morgane AU - Griesbeck M AUID- ORCID: 0000-0003-4962-102X AD - Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology, and Harvard University, Cambridge, MA, USA. FAU - Butler, Andrew AU - Butler A AUID- ORCID: 0000-0003-3608-0463 AD - New York Genome Center, New York University Center for Genomics and Systems Biology, New York, NY, USA. AD - New York University Center for Genomics and Systems Biology, New York, NY, USA. FAU - Zheng, Shiwei AU - Zheng S AUID- ORCID: 0000-0001-6682-6743 AD - New York Genome Center, New York University Center for Genomics and Systems Biology, New York, NY, USA. AD - New York University Center for Genomics and Systems Biology, New York, NY, USA. FAU - Lazo, Suzan AU - Lazo S AUID- ORCID: 0000-0002-6678-9847 AD - Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA. FAU - Jardine, Laura AU - Jardine L AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Dixon, David AU - Dixon D AUID- ORCID: 0000-0003-3346-7160 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Stephenson, Emily AU - Stephenson E AUID- ORCID: 0000-0002-4244-4019 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Nilsson, Emil AU - Nilsson E AUID- ORCID: 0000-0001-8338-8124 AD - Olink Proteomics, Watertown, MA, USA. FAU - Grundberg, Ida AU - Grundberg I AUID- ORCID: 0000-0002-8997-3962 AD - Olink Proteomics, Watertown, MA, USA. FAU - McDonald, David AU - McDonald D AUID- ORCID: 0000-0002-2410-9364 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Filby, Andrew AU - Filby A AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. FAU - Li, Weibo AU - Li W AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Center for Cancer Research, Massachusetts General Hospital, Department of Medicine, Boston, MA, USA. FAU - De Jager, Philip L AU - De Jager PL AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Program in Translational NeuroPsychiatric Genomics, Department of Neurology, Brigham and Women's Hospital and Harvard Medical School. FAU - Rozenblatt-Rosen, Orit AU - Rozenblatt-Rosen O AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. FAU - Lane, Andrew A AU - Lane AA AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. AD - Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA. FAU - Haniffa, Muzlifah AU - Haniffa M AUID- ORCID: 0000-0002-3927-2084 AD - Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK. cvillani@broadinstitute.org m.a.haniffa@newcastle.ac.uk aregev@broadinstitute.org nhacohen@mgh.harvard.edu. AD - Department of Dermatology, Royal Victoria Infirmary, Newcastle Hospitals NHS Foundation Trust, UK. FAU - Regev, Aviv AU - Regev A AUID- ORCID: 0000-0003-3293-3158 AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. cvillani@broadinstitute.org m.a.haniffa@newcastle.ac.uk aregev@broadinstitute.org nhacohen@mgh.harvard.edu. AD - Department of Biology and Koch Institute, Massachusetts Institute of Technology, Cambridge, MA, USA. AD - Howard Hughes Medical Institute, Chevy Chase, MD, USA. FAU - Hacohen, Nir AU - Hacohen N AUID- ORCID: 0000-0002-2349-2656 AD - Broad Institute of MIT and Harvard, Cambridge, MA, USA. cvillani@broadinstitute.org m.a.haniffa@newcastle.ac.uk aregev@broadinstitute.org nhacohen@mgh.harvard.edu. AD - Center for Cancer Research, Massachusetts General Hospital, Department of Medicine, Boston, MA, USA. LA - eng GR - P30 DK043351/DK/NIDDK NIH HHS/United States GR - T32 HG002295/HG/NHGRI NIH HHS/United States GR - DP2 HG009623/HG/NHGRI NIH HHS/United States GR - U19 AI082630/AI/NIAID NIH HHS/United States GR - P50 HG006193/HG/NHGRI NIH HHS/United States GR - WT_/Wellcome Trust/United Kingdom GR - P30 AI060354/AI/NIAID NIH HHS/United States GR - MR/N005872/1/MRC_/Medical Research Council/United Kingdom GR - RM1 HG006193/HG/NHGRI NIH HHS/United States GR - HHMI/Howard Hughes Medical Institute/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 SB - IM CIN - Nature. 2017 Jul 3;547(7661):125-126. PMID: 28682345 CIN - J Allergy Clin Immunol. 2018 Jun;141(6):2276-2279.e3. PMID: 29366702 MH - Adult MH - Antigen Presentation MH - Classification MH - Dendritic Cells/*classification/immunology MH - Female MH - Gene Expression Profiling MH - Humans MH - Lymphocyte Activation MH - Male MH - Monitoring, Immunologic MH - Monocytes/*classification/immunology MH - Neoplasms/immunology/pathology MH - Sequence Analysis, RNA MH - Single-Cell Analysis MH - T-Lymphocytes/*immunology MH - Transcriptome MH - Young Adult PMC - PMC5775029 MID - NIHMS910854 EDAT- 2017/04/22 06:00 MHDA- 2017/12/01 06:00 PMCR- 2018/01/19 CRDT- 2017/04/22 06:00 PHST- 2016/06/29 00:00 [received] PHST- 2017/03/07 00:00 [accepted] PHST- 2017/04/22 06:00 [entrez] PHST- 2017/04/22 06:00 [pubmed] PHST- 2017/12/01 06:00 [medline] PHST- 2018/01/19 00:00 [pmc-release] AID - 356/6335/eaah4573 [pii] AID - 10.1126/science.aah4573 [doi] PST - ppublish SO - Science. 2017 Apr 21;356(6335):eaah4573. doi: 10.1126/science.aah4573.