PMID- 28429668
OWN - NLM
STAT- MEDLINE
DCOM- 20180521
LR  - 20180521
IS  - 1875-5666 (Electronic)
IS  - 1566-5240 (Linking)
VI  - 17
IP  - 2
DP  - 2017
TI  - The Role of Mitochondria-Associated Reactive Oxygen Species in the Amyloid beta 
      Induced Production of Angiogenic Factors b y ARPE-19 Cells.
PG  - 140-148
LID - 10.2174/1566524017666170331162616 [doi]
AB  - OBJECTIVE: This study aimed to investigate the mechanisms whereby Amyloidbeta 
      (Abeta) induces the production of angiogenic factors by a human retinal pigment 
      epithelial cell line (ARPE-19) cells. METHODS: ARPE-19 cells obtained from the 
      American Type Culture Collection (ATCC) were utilized in this study. The 
      expression level of vascular endothelial growth factor (VEGF), Interleukin-8 
      (IL-8), monocyte chemoattractant protein-1 (MCP-1) and complement activation 
      fragments C3a and C5a were measured by Real-time quantitative PCR (RT-PCR) and 
      Enzyme-linked immunosorbent assay (ELISA). The production of 
      mitochondria-associated reactive oxygen species (ROS) was measured by flow 
      cytometry. RESULTS: The expression of VEGF, IL-8, MCP-1, C3a and C5a was 
      significantly increased in Abeta-treated ARPE-19 cells. Mitochondria-associated ROS 
      production was also significantly increased when exposed to Abeta. Inhibition of 
      mitochondrial ROS with Diphenyleneiodonium chloride (DPI) markedly decreased the 
      Abeta induced production of VEGF, IL-8, MCP-1, C3a and C5a by ARPE-19 cells. 
      Anti-C3a or anti-C5a neutralizing antibodies did not have a detectable influence 
      on the secretion of VEGF, IL-8 and MCP-1 by ARPE-19 cells upon stimulation with 
      Abeta. CONCLUSION: Our results support the hypothesis that Abeta is involved in the 
      pathogenesis of choroidal neovascularization (CNV) formation by promoting the 
      production of the angiogenic cytokines VEGF, IL-8 and MCP-1 by RPE cells. 
      Mitochondrial ROS was shown to play a role in the regulation of Abeta induced 
      expression of these cytokines.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Wu, L
AU  - Wu L
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Tan, X
AU  - Tan X
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Liang, L
AU  - Liang L
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Yu, H
AU  - Yu H
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Wang, C
AU  - Wang C
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Zhang, D
AU  - Zhang D
AD  - The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab 
      of Ophthalmology, Chongqing Eye Institute, Chongqing. China.
FAU - Kijlstra, A
AU  - Kijlstra A
AD  - Eye Research Institute Maastricht, Department of Ophthalmology, University 
      Hospital Maastricht, Maastricht. Netherlands.
FAU - Yang, P
AU  - Yang P
AD  - The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, 
      Chongqing 400016, People's. China.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Curr Mol Med
JT  - Current molecular medicine
JID - 101093076
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 80295-42-7 (Complement C3a)
RN  - 80295-54-1 (Complement C5a)
SB  - IM
MH  - Amyloid beta-Peptides/*metabolism
MH  - Angiogenesis Inducing Agents/*metabolism
MH  - Biomarkers
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Complement C3a/immunology
MH  - Complement C5a/immunology
MH  - Humans
MH  - Interleukin-8/genetics/metabolism
MH  - Mitochondria/genetics/*metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Retinal Pigment Epithelium/metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - Amyloid-beta
OT  - age-related macular degeneration
OT  - choroidal neovascularization
OT  - drusen
OT  - mitochondria-associated reactive oxygen species
OT  - retinal pigment epithelium
EDAT- 2017/04/22 06:00
MHDA- 2018/05/22 06:00
CRDT- 2017/04/22 06:00
PHST- 2016/11/23 00:00 [received]
PHST- 2017/02/18 00:00 [revised]
PHST- 2017/02/21 00:00 [accepted]
PHST- 2017/04/22 06:00 [pubmed]
PHST- 2018/05/22 06:00 [medline]
PHST- 2017/04/22 06:00 [entrez]
AID - CMM-EPUB-82604 [pii]
AID - 10.2174/1566524017666170331162616 [doi]
PST - ppublish
SO  - Curr Mol Med. 2017;17(2):140-148. doi: 10.2174/1566524017666170331162616.