PMID- 28430606
OWN - NLM
STAT- MEDLINE
DCOM- 20180416
LR  - 20181113
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 20
DP  - 2017 May 16
TI  - Ketamine administered pregnant rats impair learning and memory in offspring via 
      the CREB pathway.
PG  - 32433-32449
LID - 10.18632/oncotarget.15405 [doi]
AB  - Ketamine has been reported to impair the capacity for learning and memory. This 
      study examined whether these capacities were also altered in the offspring and 
      investigated the role of the CREB signaling pathway in pregnant rats, subjected 
      to ketamine-induced anesthesia. On the 14th day of gestation (P14), female rats 
      were anesthetized for 3 h via intravenous ketamine injection (200 mg/Kg). Morris 
      water maze task, contextual and cued fear conditioning, and olfactory tasks were 
      executed between the 25th to 30th day after birth (B25-30) on rat pups, and rats 
      were sacrificed on B30. Nerve density and dendritic spine density were examined 
      via Nissl's and Golgi staining. Simultaneously, the contents of 
      Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII), p-CaMKII, CaMKIV, p-CaMKIV, 
      Extracellular Regulated Protein Kinases (ERK), p-ERK, Protein Kinase A (PKA), 
      p-PKA, cAMP-Response Element Binding Protein (CREB), p-CREB, and Brain Derived 
      Neurotrophic Factor (BDNF) were detected in the hippocampus. We pretreated PC12 
      cells with both PKA inhibitor (H89) and ERK inhibitor (SCH772984), thus detecting 
      levels of ERK, p-ERK, PKA, p-PKA, p-CREB, and BDNF. The results revealed that 
      ketamine impaired the learning ability and spatial as well as conditioned memory 
      in the offspring, and significantly decreased the protein levels of ERK, p-ERK, 
      PKA, p-PKA, p-CREB, and BDNF. We found that ERK and PKA (but not CaMKII or 
      CaMKIV) have the ability to regulate the CREB-BDNF pathway during 
      ketamine-induced anesthesia in pregnant rats. Furthermore, ERK and PKA are 
      mutually compensatory for the regulation of the CREB-BDNF pathway.
FAU - Li, Xinran
AU  - Li X
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Guo, Cen
AU  - Guo C
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Li, Yanan
AU  - Li Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Li, Lina
AU  - Li L
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Wang, Yuxin
AU  - Wang Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Zhang, Yiming
AU  - Zhang Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Li, Yue
AU  - Li Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Liu, Wenhan
AU  - Liu W
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Gao, Li
AU  - Gao L
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 690G0D6V8H (Ketamine)
SB  - IM
MH  - Animals
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Female
MH  - Humans
MH  - Ketamine/*adverse effects
MH  - Learning/*drug effects
MH  - Male
MH  - Memory/*drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction
PMC - PMC5464800
OTO - NOTNLM
OT  - CREB pathway
OT  - Neuroscience
OT  - ketamine
OT  - learning and memory
OT  - pregnant rats
OT  - rat offspring
COIS- CONFLICTS OF INTEREST There is no conflict of interest.
EDAT- 2017/04/22 06:00
MHDA- 2018/04/17 06:00
PMCR- 2017/05/16
CRDT- 2017/04/22 06:00
PHST- 2016/12/21 00:00 [received]
PHST- 2017/01/27 00:00 [accepted]
PHST- 2017/04/22 06:00 [pubmed]
PHST- 2018/04/17 06:00 [medline]
PHST- 2017/04/22 06:00 [entrez]
PHST- 2017/05/16 00:00 [pmc-release]
AID - 15405 [pii]
AID - 10.18632/oncotarget.15405 [doi]
PST - ppublish
SO  - Oncotarget. 2017 May 16;8(20):32433-32449. doi: 10.18632/oncotarget.15405.