PMID- 28432146
OWN - NLM
STAT- MEDLINE
DCOM- 20170922
LR  - 20180127
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 198
IP  - 11
DP  - 2017 Jun 1
TI  - C1q-Dependent Dendritic Cell Cross-Presentation of In Vivo-Formed 
      Antigen-Antibody Complexes.
PG  - 4235-4243
LID - 10.4049/jimmunol.1602169 [doi]
AB  - Dendritic cells (DCs) are specialized in Ag engulfment via a wide variety of 
      uptake receptors on their cell surface. In the present study we investigated Ag 
      uptake and presentation of in vivo-formed Ag-Ab complexes by i.v. injecting mice 
      with Ag-specific Abs followed by the cognate Ag. We show by this natural 
      Ab-mediated Ag targeting system that uptake by splenic APC subsets is severely 
      hampered in mice lacking complement factor C1q (C1qa(-/-)). Moreover, no 
      detectable Ag cross-presentation by CD8alpha(+) DCs from C1qa(-/-) mice was found. On 
      the contrary, Ag uptake was not hampered by APCs in FcgammaRI/II/III/IV-deficient 
      (FcgammaR quadruple(-/-)) mice, and the cross-presentation ability of CD8alpha(+) DCs was 
      not affected. In conclusion, we show that C1q rather than FcgammaRs controls the 
      Ab-mediated Ag uptake and its presentation by spleen APC subsets to T cells.
CI  - Copyright (c) 2017 by The American Association of Immunologists, Inc.
FAU - Ho, Nataschja I
AU  - Ho NI
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, 2300 RC Leiden, the Netherlands.
FAU - Camps, Marcel G M
AU  - Camps MGM
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, 2300 RC Leiden, the Netherlands.
FAU - de Haas, Edwin F E
AU  - de Haas EFE
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, 2300 RC Leiden, the Netherlands.
FAU - Trouw, Leendert A
AU  - Trouw LA
AUID- ORCID: 0000-0001-5186-2290
AD  - Department of Rheumatology, Leiden University Medical Center, 2300 RC Leiden, the 
      Netherlands; and.
FAU - Verbeek, J Sjef
AU  - Verbeek JS
AD  - Department of Human Genetics, Leiden University Medical Center, 2300 RC Leiden, 
      the Netherlands.
FAU - Ossendorp, Ferry
AU  - Ossendorp F
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, 2300 RC Leiden, the Netherlands; f.a.ossendorp@lumc.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170421
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (CD8 Antigens)
RN  - 0 (Receptors, IgG)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Adaptive Immunity
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigen-Antibody Complex/*immunology
MH  - CD8 Antigens/immunology
MH  - Complement C1q/deficiency/genetics/*immunology
MH  - Cross-Priming
MH  - Dendritic Cells/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, IgG/immunology
EDAT- 2017/04/23 06:00
MHDA- 2017/09/25 06:00
CRDT- 2017/04/23 06:00
PHST- 2016/12/27 00:00 [received]
PHST- 2017/03/29 00:00 [accepted]
PHST- 2017/04/23 06:00 [pubmed]
PHST- 2017/09/25 06:00 [medline]
PHST- 2017/04/23 06:00 [entrez]
AID - jimmunol.1602169 [pii]
AID - 10.4049/jimmunol.1602169 [doi]
PST - ppublish
SO  - J Immunol. 2017 Jun 1;198(11):4235-4243. doi: 10.4049/jimmunol.1602169. Epub 2017 
      Apr 21.