PMID- 28432436
OWN - NLM
STAT- MEDLINE
DCOM- 20180307
LR  - 20181113
IS  - 1432-1750 (Electronic)
IS  - 0341-2040 (Linking)
VI  - 195
IP  - 3
DP  - 2017 Jun
TI  - Chemokine Involvement in Lung Injury Secondary to Ischaemia/Reperfusion.
PG  - 333-340
LID - 10.1007/s00408-017-0001-x [doi]
AB  - INTRODUCTION: During transplant surgeries, the lung experiences an 
      ischaemia-reperfusion (I/R)-induced damage identified as a significant cause of 
      morbidity and mortality. However, the mechanisms by which I/R induces leucocyte 
      accumulation and subsequent tissue damage in lung surgeries remain unknown. 
      Therefore, the present study aims to assess the role of monocyte chemotactic 
      protein 1 (MCP-1) and macrophage inflammatory protein 2 (MIP-2) in leucocyte 
      chemotaxis related to lung injury secondary to I/R. METHODS: Six pigs were 
      subjected to an orthotopic left caudal lobe lung transplantation with a 
      subsequent 60-min graft reperfusion (Transplant group). In addition, six animals 
      underwent to sham surgery (Sham Group). Plasma samples and lung biopsies were 
      collected before the beginning of pneumonectomy, before starting the reperfusion, 
      and 30 min and 60 min after the beginning of the reperfusion. Plasma levels of 
      intercellular adhesion molecule 1 (ICAM-1) and lung expressions of MCP-1, MIP-2, 
      myeloperoxidase (MPO), and lung oedema were measured. RESULTS: Lung I/R caused 
      substantial damage observed as pulmonary oedema. The oedema was evident after the 
      ischemic insult and increased after reperfusion. After reperfusion, increased 
      levels of MPO were observed which suggests an activation and infiltration of 
      neutrophils into the lung tissue. After 30 min of reperfusion, MCP-1, MIP-2, and 
      ICAM-1 levels were significantly increased compared to prepneumonectomy levels 
      (p < 0.05) and a further increase was observed after 60 min of reperfusion 
      (p < 0.05). CONCLUSION: The present study demonstrates that activated 
      neutrophils, as well as MCP-1, MIP-2, and ICAM-1, are involved in inflammatory 
      response induced by ischaemia-reperfusion-induced lung injury.
FAU - Rancan, Lisa
AU  - Rancan L
AUID- ORCID: 0000-0003-1556-192X
AD  - Department of Biochemistry and Molecular Biology III, School of Medicine, 
      Complutense University of Madrid, Av.da Complutense s/n, 28040, Madrid, Spain. 
      lisaranc@ucm.es.
FAU - Paredes, Sergio D
AU  - Paredes SD
AD  - Department of Physiology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Huerta, Luis
AU  - Huerta L
AD  - Service of Thoracic Surgery, Gregorio Maranon University General Hospital, 
      Madrid, Spain.
FAU - Casanova, Javier
AU  - Casanova J
AD  - Service of Anaesthesiology and Rehabilitation, Gregorio Maranon University 
      General Hospital, Madrid, Spain.
FAU - Guzman, Jorge
AU  - Guzman J
AD  - Department of Biochemistry and Molecular Biology III, School of Medicine, 
      Complutense University of Madrid, Av.da Complutense s/n, 28040, Madrid, Spain.
FAU - Garutti, Ignacio
AU  - Garutti I
AD  - Service of Anaesthesiology and Rehabilitation, Gregorio Maranon University 
      General Hospital, Madrid, Spain.
FAU - Gonzalez-Aragoneses, Federico
AU  - Gonzalez-Aragoneses F
AD  - Service of Thoracic Surgery, Gregorio Maranon University General Hospital, 
      Madrid, Spain.
FAU - Simon, Carlos
AU  - Simon C
AD  - Service of Thoracic Surgery, Gregorio Maranon University General Hospital, 
      Madrid, Spain.
FAU - Vara, Elena
AU  - Vara E
AD  - Department of Biochemistry and Molecular Biology III, School of Medicine, 
      Complutense University of Madrid, Av.da Complutense s/n, 28040, Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170421
PL  - United States
TA  - Lung
JT  - Lung
JID - 7701875
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Acute Lung Injury/*blood/etiology/metabolism/pathology
MH  - Animals
MH  - Chemokine CCL2/*blood/metabolism
MH  - Chemokine CXCL2/*blood/metabolism
MH  - Ischemia/complications
MH  - Lung Transplantation/adverse effects
MH  - Peroxidase/metabolism
MH  - Pneumonectomy/adverse effects
MH  - Pulmonary Edema/*etiology
MH  - Reperfusion/adverse effects
MH  - Reperfusion Injury/*blood/etiology/metabolism/pathology
MH  - Swine
OTO - NOTNLM
OT  - ICAM-1
OT  - Ischaemia-reperfusion injury
OT  - Lung
OT  - MCP-2
OT  - MIP-1
EDAT- 2017/04/23 06:00
MHDA- 2018/03/08 06:00
CRDT- 2017/04/23 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/03/27 00:00 [accepted]
PHST- 2017/04/23 06:00 [pubmed]
PHST- 2018/03/08 06:00 [medline]
PHST- 2017/04/23 06:00 [entrez]
AID - 10.1007/s00408-017-0001-x [pii]
AID - 10.1007/s00408-017-0001-x [doi]
PST - ppublish
SO  - Lung. 2017 Jun;195(3):333-340. doi: 10.1007/s00408-017-0001-x. Epub 2017 Apr 21.