PMID- 28433894
OWN - NLM
STAT- MEDLINE
DCOM- 20180227
LR  - 20221207
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 96
DP  - 2017 Aug
TI  - A functional SNP MCP-1 (-2518A/G) predispose to renal disorder in Indian Systemic 
      Lupus Erythematosus patients.
PG  - 189-194
LID - S1043-4666(17)30110-2 [pii]
LID - 10.1016/j.cyto.2017.04.016 [doi]
AB  - Systemic Lupus Erythematosus (SLE) is a clinically heterogeneous chronic, 
      inflammatory autoimmune disorder that affects multiple organs where exact 
      etiology of the disease is not yet clearly understood. Various evidences suggest 
      that genetic polymorphisms in inflammatory mediators like cytokines and 
      chemokines may influence development of the disease. Here, we investigated 
      whether functional polymorphism at the Monocyte Chemoattractant Protein-1 (MCP-1) 
      regulatory region associates with disease phenotype in Indian SLE patients. This 
      case control study included 200 SLE patients and 201 ethnically matched healthy 
      controls. Genotyping of MCP-1 (-2518 A/G) polymorphism was performed using 
      PCR-RFLP method. Serum MCP-1 levels were detected by bead-based multiplex 
      immunoassay. Serum MCP-1 levels were found to be higher in patients compared with 
      healthy individuals (p<0.0001). A significant difference for MCP-1G allele 
      frequency (OR=1.9, 95%CI=1.4-2.6, p<0.0001) was observed among SLE patients 
      against healthy individuals. A significant difference in the distribution of 
      MCP-1 -2518GG (OR=3.0, 95%CI=1.4-6.7, p=0.0041) and AG+GG genotypes (OR=2.0, 
      95%CI=1.4-3.0, p=0.0005) was also noted among SLE patients when compared with 
      healthy individuals. A significant association was observed between A/G and G/G 
      versus A/A genotypes with renal manifestations (p<0.0001, Pc<0.001). Serum MCP-1 
      levels in active LN patients were found to be significantly higher than inactive 
      LN (p=0.0059), mild LN (p=0.0061) as well as non-LN patients (p=0.0001). These 
      findings suggest that -2518G allele of MCP-1 -2518 A/G polymorphism is associated 
      with renal disorders and may influence MCP-1 gene expression among Indian SLE 
      patients.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Umare, Vinod D
AU  - Umare VD
AD  - National Institute of Immunohaematology, Indian Council of Medical Research, 
      Mumbai, Maharashtra 400012, India.
FAU - Pradhan, Vandana D
AU  - Pradhan VD
AD  - National Institute of Immunohaematology, Indian Council of Medical Research, 
      Mumbai, Maharashtra 400012, India.
FAU - Rajadhyaksha, Anjali G
AU  - Rajadhyaksha AG
AD  - Department of Rheumatology, King Edward Memorial Hospital, Parel, Mumbai, 
      Maharashtra 400012, India.
FAU - Ghosh, Kanjaksha
AU  - Ghosh K
AD  - National Institute of Immunohaematology, Indian Council of Medical Research, 
      Mumbai, Maharashtra 400012, India.
FAU - Nadkarni, Anita H
AU  - Nadkarni AH
AD  - National Institute of Immunohaematology, Indian Council of Medical Research, 
      Mumbai, Maharashtra 400012, India. Electronic address: anitahnadkarni@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20170421
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Adult
MH  - Asian People
MH  - Case-Control Studies
MH  - Chemokine CCL2/blood/*genetics
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - India
MH  - Kidney/*physiopathology
MH  - Lupus Erythematosus, Systemic/ethnology/*genetics
MH  - Lupus Nephritis/ethnology/*genetics
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Young Adult
OTO - NOTNLM
OT  - CCL2
OT  - Chemokine
OT  - Indian SLE
OT  - MCP-1
OT  - PCR-RFLP
OT  - Polymorphism
EDAT- 2017/04/24 06:00
MHDA- 2018/02/28 06:00
CRDT- 2017/04/24 06:00
PHST- 2016/10/26 00:00 [received]
PHST- 2017/03/14 00:00 [revised]
PHST- 2017/04/14 00:00 [accepted]
PHST- 2017/04/24 06:00 [pubmed]
PHST- 2018/02/28 06:00 [medline]
PHST- 2017/04/24 06:00 [entrez]
AID - S1043-4666(17)30110-2 [pii]
AID - 10.1016/j.cyto.2017.04.016 [doi]
PST - ppublish
SO  - Cytokine. 2017 Aug;96:189-194. doi: 10.1016/j.cyto.2017.04.016. Epub 2017 Apr 21.