PMID- 28434228
OWN - NLM
STAT- MEDLINE
DCOM- 20170608
LR  - 20170608
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 65
IP  - 18
DP  - 2017 May 10
TI  - Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by 
      Cupriavidus gilardii T-1.
PG  - 3711-3720
LID - 10.1021/acs.jafc.7b00544 [doi]
AB  - Phytotoxicity and environmental pollution of residual herbicides have caused much 
      public concern during the past several decades. An indigenous bacterial strain 
      capable of degrading 2,4-dichlorophenoxyacetic acid (2,4-D), designated T-1, was 
      isolated from soybean field soil and identified as Cupriavidus gilardii. Strain 
      T-1 degraded 2,4-D 3.39 times more rapidly than the model strain Cupriavidus 
      necator JMP134. T-1 could also efficiently degrade 2-methyl-4-chlorophenoxyacetic 
      acid (MCPA), MCPA isooctyl ester, and 2-(2,4-dichlorophenoxy)propionic acid 
      (2,4-DP). Suitable conditions for 2,4-D degradation were pH 7.0-9.0, 37-42  degrees C, 
      and 4.0 mL of inoculums. Degradation of 2,4-D was concentration-dependent. 2,4-D 
      was degraded to 2,4-dichlorophenol (2,4-DCP) by cleavage of the ether bond and 
      then to 3,5-dichlorocatechol (3,5-DCC) via hydroxylation, followed by 
      ortho-cleavage to cis-2-dichlorodiene lactone (CDL). The metabolites 2,4-DCP or 
      3,5-DCC at 10 mg L(-1) were completely degraded within 16 h. Fast degradation of 
      2,4-D and its analogues highlights the potential for use of C. gilardii T-1 in 
      bioremediation of phenoxyalkanoic acid herbicides.
FAU - Wu, Xiangwei
AU  - Wu X
AUID- ORCID: 0000-0003-3589-4051
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Wang, Wenbo
AU  - Wang W
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Liu, Junwei
AU  - Liu J
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Pan, Dandan
AU  - Pan D
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Tu, Xiaohui
AU  - Tu X
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Lv, Pei
AU  - Lv P
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Wang, Yi
AU  - Wang Y
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Cao, Haiqun
AU  - Cao H
AUID- ORCID: 0000-0001-9381-8896
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Wang, Yawen
AU  - Wang Y
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
FAU - Hua, Rimao
AU  - Hua R
AUID- ORCID: 0000-0002-5601-6568
AD  - College of Resources and Environment, Key Laboratory of Agri-food Safety of Anhui 
      Province, Anhui Agricultural University , Hefei 230036, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170501
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Herbicides)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/chemistry/*metabolism
MH  - Biodegradation, Environmental
MH  - Cupriavidus/chemistry/genetics/isolation & purification/*metabolism
MH  - Herbicides/chemistry/*metabolism
MH  - Molecular Structure
MH  - Soil Microbiology
OTO - NOTNLM
OT  - 2,4-D
OT  - Cupriavidus gilardii
OT  - biodegradation
OT  - degradation pathway
OT  - degradation rate
OT  - phenoxyalkanoic acid herbicide
EDAT- 2017/04/25 06:00
MHDA- 2017/06/09 06:00
CRDT- 2017/04/25 06:00
PHST- 2017/04/25 06:00 [pubmed]
PHST- 2017/06/09 06:00 [medline]
PHST- 2017/04/25 06:00 [entrez]
AID - 10.1021/acs.jafc.7b00544 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2017 May 10;65(18):3711-3720. doi: 10.1021/acs.jafc.7b00544. 
      Epub 2017 May 1.