PMID- 28435194
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 0972-8988 (Print)
IS  - 2231-0916 (Electronic)
IS  - 0972-8988 (Linking)
VI  - 10
IP  - 3
DP  - 2017 Mar
TI  - Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) 
      disease virus from different outbreaks in desi chickens.
PG  - 319-323
LID - 10.14202/vetworld.2017.319-323 [doi]
AB  - AIM: Diagnosis of velogenic viscerotropic Ranikhet disease from six different 
      flocks of desi chicken in and around Mumbai by gross and histopathological 
      examination, isolation of virus and molecular methods. MATERIALS AND METHODS: A 
      total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six 
      different flocks of adult desi chicken were subjected to necropsy examination for 
      diagnosis of the disease during the span of a year (2014-2015). After thorough 
      gross examination, the tissue samples were collected and processed for virus 
      isolation and histopathological examination. The 20% tissue homogenate was 
      inoculated into 9-day-old specific pathogen free (SPF) embryonated eggs. Mean 
      death time (MDT) of embryos after inoculation and intracerebral pathogenicity 
      index (ICPI) were used to judge velogenic nature of the virus. Newcastle disease 
      virus (NDV) was isolated from six cases and confirmed by reverse 
      transcriptase-polymerase chain reaction (RT-PCR) targeting the partial fusion 
      protein gene of the viral genome. RESULTS: A total of 25 carcasses (varying 
      between 2 and 6 carcasses from each flock) of six different flocks of desi 
      chicken were presented for postmortem examination to Department of Veterinary 
      Pathology, Bombay Veterinary College, Parel, Mumbai during 2014-2015. The gross 
      and histopathological examination revealed lesions suggestive of viscerotropic 
      velogenic form of the Newcastle disease (ND). The 20% tissue homogenate was 
      inoculated into 9-day-old embryonated eggs from SPF chicken. NDV was isolated 
      from six cases and confirmed by RT-PCR targeting the partial fusion protein gene. 
      MDT of all the isolates was <60 h which indicated velogenic nature of the virus. 
      ICPI of the isolates ranged between the 1.63 and 1.78. In four out of six 
      outbreaks concurrent moderate to heavy infection of Ascardii galli in one flock 
      and Railetina spp. in three flocks was also noted. In this study, viscerotropic 
      velogenic form of ND was confirmed in all six outbreaks by gross and microscopic 
      examination, virus isolation and RT-PCR. CONCLUSIONS: In this study, 
      viscerotropic velogenic form of ND was confirmed in all six outbreaks by gross 
      and microscopic examination, virus isolation and RT-PCR. Nowadays, vaccine 
      strains Lasota, B1 and F strains are used widely in India to control the 
      infection of NDV. However, virulent NDV strains are still isolated frequently in 
      the birds under backyard and also in commercial venture which demonstrates that 
      NDV remains an on-going threat to commercial as well as backyard poultry flocks.
FAU - Dhaygude, V S
AU  - Dhaygude VS
AD  - Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai, 
      Maharashtra, India.
FAU - Sawale, G K
AU  - Sawale GK
AD  - Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai, 
      Maharashtra, India.
FAU - Chawak, M M
AU  - Chawak MM
AD  - Department of Veterinary Pathology, Poultry Diagnostic and Research Centre, Lono 
      Kalbhor, Pune, Maharashtra, India.
FAU - Bulbule, N R
AU  - Bulbule NR
AD  - Department of Veterinary Pathology, Poultry Diagnostic and Research Centre, Lono 
      Kalbhor, Pune, Maharashtra, India.
FAU - Moregaonkar, S D
AU  - Moregaonkar SD
AD  - Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai, 
      Maharashtra, India.
FAU - Gavhane, D S
AU  - Gavhane DS
AD  - Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai, 
      Maharashtra, India.
LA  - eng
PT  - Journal Article
DEP - 20170317
PL  - India
TA  - Vet World
JT  - Veterinary world
JID - 101504872
PMC - PMC5387659
OTO - NOTNLM
OT  - Newcastle disease virus
OT  - mean death time
OT  - reverse transcriptase polymerase chain reaction
OT  - specific pathogen free
OT  - velogenic
EDAT- 2017/04/25 06:00
MHDA- 2017/04/25 06:01
PMCR- 2017/03/01
CRDT- 2017/04/25 06:00
PHST- 2016/11/26 00:00 [received]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/04/25 06:00 [entrez]
PHST- 2017/04/25 06:00 [pubmed]
PHST- 2017/04/25 06:01 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - 10.14202/vetworld.2017.319-323 [doi]
PST - ppublish
SO  - Vet World. 2017 Mar;10(3):319-323. doi: 10.14202/vetworld.2017.319-323. Epub 2017 
      Mar 17.