PMID- 28436303
OWN - NLM
STAT- MEDLINE
DCOM- 20180727
LR  - 20180801
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 25
IP  - 1
DP  - 2018 Jan
TI  - Choline Supplementation During Pregnancy Protects Against Gestational 
      Lipopolysaccharide-Induced Inflammatory Responses.
PG  - 74-85
LID - 10.1177/1933719117702247 [doi]
AB  - OBJECTIVES: To estimate the effects and mechanisms of choline, an essential 
      nutrient and a selective alpha7 nicotinic acetylcholine receptor (alpha7nAChR) agonist, 
      on the prevention of symptoms and the effects on the cholinergic 
      anti-inflammatory pathways (CAP) in a lipopolysaccharide (LPS)-induced 
      inflammatory response in a rat model. METHODS: Inflammation was induced by LPS 
      treatment (1.0 mug LPS/kg body weight) on gestational day (GD) 14. Nonpregnant and 
      pregnant Sprague Dawley rats were placed on a normal choline diet (1.1 g/kg) or 
      supplemented choline diet (5.0 g/kg) from GDs 1 to 20. Systolic blood pressure 
      (SBP), urinary albumin, and pregnancy outcomes were recorded. On GD 20, serum and 
      placentas were assayed for cytokines. Western blots were used to determine the 
      expression of placenta alpha7nAChR and components of the alpha7nAChR-CAP, including 
      nuclear factor-kappaB (NF-kappaB) and protein kinase B (AKT). Immunohistochemistry was 
      used to localize placental sites for the p65 subunit of NF-kappaB. RESULTS: 
      Lipopolysaccharide significantly increased SBP and urinary albumin and decreased 
      pregnancy outcomes, and these effects were partially reversed by higher choline 
      treatment. Choline supplementation also significantly attenuated the LPS-induced 
      increase in serum and placental inflammatory cytokines, decreased the expression 
      of placental alpha7nAChR, lowered the activation of NF-kappaB signaling in placenta 
      mononuclear cells, and inhibited placental AKT phosphorylation. CONCLUSION: This 
      study confirms that LPS induces inflammatory conditions in pregnant rats and 
      shows that choline supplementation protects against the inflammatory symptoms 
      through its action on alpha7nAChR and CAP. These observations have important 
      implications for the prevention and treatment of inflammatory responses 
      associated with pregnancy.
FAU - Zhang, Min
AU  - Zhang M
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Han, Xinjia
AU  - Han X
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Bao, Juejie
AU  - Bao J
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Yang, Jinying
AU  - Yang J
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Shi, Shao-Qing
AU  - Shi SQ
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Garfield, Robert E
AU  - Garfield RE
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
FAU - Liu, Huishu
AU  - Liu H
AD  - 1 Department of Obstetrics, Preterm Birth Prevention and Treatment Research Unit, 
      Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 
      Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170423
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protective Agents)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animals
MH  - Choline/administration & dosage/*therapeutic use
MH  - Cytokines/metabolism
MH  - *Dietary Supplements
MH  - Female
MH  - Inflammation/chemically induced/*drug therapy/metabolism
MH  - Lipopolysaccharides
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Protective Agents/administration & dosage/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - choline
OT  - cholinergic anti-inflammatory pathway
OT  - cytokines
OT  - inflammation
OT  - preeclampsia
OT  - pregnancy
OT  - preterm birth
OT  - alpha7nAChR
EDAT- 2017/04/25 06:00
MHDA- 2018/07/28 06:00
CRDT- 2017/04/25 06:00
PHST- 2017/04/25 06:00 [pubmed]
PHST- 2018/07/28 06:00 [medline]
PHST- 2017/04/25 06:00 [entrez]
AID - 10.1177/1933719117702247 [doi]
PST - ppublish
SO  - Reprod Sci. 2018 Jan;25(1):74-85. doi: 10.1177/1933719117702247. Epub 2017 Apr 
      23.