PMID- 2843763
OWN - NLM
STAT- MEDLINE
DCOM- 19881018
LR  - 20131121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 2
IP  - 6
DP  - 1988 Jun
TI  - Unspecific and sequence-specific deoxyribonucleic acid binding of the partially 
      purified human progesterone receptor.
PG  - 571-8
AB  - The progesterone receptor (PR) was partially purified from T47D human breast 
      cancer cells by sequential chromatography on phosphocellulose, heparin-Sepharose, 
      and DNA-cellulose. Heparin-Sepharose chromatography resulted in an efficient 
      conversion of the receptor to a DNA-binding form (activation) since more than 85% 
      of the 3H-R5020 labeled eluate from heparin-Sepharose was retained on 
      DNA-cellulose and since the cytosolic 8S receptor was converted to a 4S moiety 
      after chromatography on heparin-Sepharose. The 3H-R5020 labeled human PR eluted 
      from DNA-cellulose as a single symmetrical peak at 0.2 M NaCl; after 
      photoaffinity labeling and sodium dodecyl sulfate-polyacrylamide gel 
      electrophoresis, this species was shown to consist of about equal amounts of two 
      proteins of Mr approximately equal to 96,000 and 120,000 (the so called A- and 
      B-subunits, respectively). This partially purified receptor preparation (SA 490 
      pmol/mg protein) did not contain any glucocorticoid receptor (GR) as shown by 
      immunoblotting with a monoclonal antirat GR antibody that cross-reacts with the 
      human GR. Therefore, this preparation was used to compare the specific 
      DNA-binding properties of the human PR with those of the purified rat GR. The 
      human PR bound specifically to the promoter region of mouse mammary tumor virus 
      (MMTV) at a molar ratio between receptor and DNA similar to the molar ratio 
      between GR and DNA needed for binding of rat GR to MMTV, indicating that the PR 
      was purified in a biologically active form.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Berkenstam, A
AU  - Berkenstam A
AD  - Department of Pathology, Karolinska Institutet, Huddinge University Hospital, 
      Sweden.
FAU - Glaumann, H
AU  - Glaumann H
FAU - Gustafsson, J A
AU  - Gustafsson JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Affinity Labels)
RN  - 0 (DNA, Viral)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Progesterone)
RN  - 9007-49-2 (DNA)
RN  - 9XE0V2SQYX (Promegestone)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Affinity Labels
MH  - Animals
MH  - Breast Neoplasms/*analysis
MH  - Centrifugation, Density Gradient
MH  - Chromatography
MH  - Cytosol/analysis
MH  - DNA/*metabolism
MH  - DNA, Viral/metabolism
MH  - Deoxyribonuclease I
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Photochemistry
MH  - Promegestone/metabolism
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Receptors, Glucocorticoid/metabolism
MH  - Receptors, Progesterone/isolation & purification/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
AID - 10.1210/mend-2-6-571 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1988 Jun;2(6):571-8. doi: 10.1210/mend-2-6-571.