PMID- 2843766
OWN - NLM
STAT- MEDLINE
DCOM- 19881021
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 201
IP  - 1
DP  - 1988 Sep
TI  - Mutational spectrum and recombinogenic effects induced by aminofluorene adducts 
      in bacteriophage M13.
PG  - 203-12
AB  - Double-stranded replicative form (RFI) DNA of bacteriophage M13 strain M13mp10 
      which carries partial lacZ gene has been modified in vitro to various extents 
      with N-hydroxy-2-amino-fluorene (N-OH-AF) and then transfected into E. coli 
      cells. High-performance liquid chromatography (HPLC) analysis results demonstrate 
      that the sole adduct (95%) formed in modified DNA is 
      N-(deoxyguanosine-8-yl)-2-aminofluorene (dG-C8-AF). Approximately 20 adducts per 
      RFI molecule constitute 1 lethal event when plaque-forming ability is assayed on 
      E. coli cells which have received no prior SOS induction. The mutagenicity of 
      dG-C8-AF adducts was assayed by measuring loss of beta-galactosidase activity as 
      a function of adducts per molecule. A dose-dependent increase in Lac- mutants was 
      observed, with a 4-fold increase in mutants per survivor at 30 adducts/molecule. 
      The mutations produced, characterized by DNA sequencing, occur predominantly at 
      either G or C positions different from those observed in the spontaneous mutant 
      spectrum. Restriction-mapping results show that in our assay system, dG-C8-AF 
      adducts induce a previously unreported recombinogenic activity.
FAU - Ross, J
AU  - Ross J
AD  - University of Texas System Cancer Center, Science Park-Research Division, 
      Smithville 78957.
FAU - Doisy, R
AU  - Doisy R
FAU - Tang, M S
AU  - Tang MS
LA  - eng
GR  - ES 03124/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (2-aminofluorene-DNA complex)
RN  - 0 (Carcinogens)
RN  - 0 (DNA Adducts)
RN  - 0 (DNA, Viral)
RN  - 0 (Fluorenes)
RN  - 0 (Mutagens)
RN  - 1D7E8EIA7K (N-(deoxyguanosin-8-yl)-2-aminofluorene)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GGCC-specific type II deoxyribonucleases)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - Bacteriophages/*genetics
MH  - Base Sequence
MH  - Carcinogens
MH  - Chromatography, High Pressure Liquid
MH  - DNA/*pharmacology
MH  - *DNA Adducts
MH  - DNA Restriction Enzymes
MH  - DNA, Viral/*drug effects/genetics
MH  - Deoxyguanosine/analogs & derivatives/pharmacology
MH  - *Deoxyribonucleases, Type II Site-Specific
MH  - Fluorenes/*pharmacology
MH  - Molecular Sequence Data
MH  - Mutagens
MH  - *Mutation
MH  - Recombination, Genetic/*drug effects
MH  - Transfection
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 0027-5107(88)90127-3 [pii]
AID - 10.1016/0027-5107(88)90127-3 [doi]
PST - ppublish
SO  - Mutat Res. 1988 Sep;201(1):203-12. doi: 10.1016/0027-5107(88)90127-3.