PMID- 28438528
OWN - NLM
STAT- MEDLINE
DCOM- 20171106
LR  - 20181202
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 249
DP  - 2017 Aug 1
TI  - Molecular characterization of Kiss2 receptor and in vitro effects of Kiss2 on 
      reproduction-related gene expression in the hypothalamus of half-smooth tongue 
      sole (Cynoglossus semilaevis).
PG  - 55-63
LID - S0016-6480(17)30264-2 [pii]
LID - 10.1016/j.ygcen.2017.04.006 [doi]
AB  - Kisspeptin (Kiss) and its receptor, KissR (previously known as GPR54), play a 
      critical role in the control of reproduction and puberty onset in mammals. 
      Additionally, a number of studies have provided evidence of the existence of 
      multiple Kiss/KissR systems in teleosts, but the physiological relevance and 
      functions of these kisspeptin forms (Kiss1 and Kiss2) still remain to be 
      investigated. To this end, we examined the direct actions of Kiss2 on 
      hypothalamic functions in the half-smooth tongue sole (Cynoglossus semilaevis), a 
      representative species of the order Pleuronectiformes. As a first step, the 
      full-length cDNA for kiss2r was identified and kiss2r transcripts were shown to 
      be widely expressed in various tissues, notably in the brain of tongue sole. 
      Then, the effects of Kiss2 decapeptide on reproduction-related gene expression 
      were evaluated using a primary hypothalamus culture system. Our results showed 
      that neither gnrh2 nor gnrh3 mRNA levels were altered by Kiss2. However, Kiss2 
      significantly increased the amounts of gnih and kiss2 mRNAs. In contrast, Kiss2 
      elicited an evident inhibitory effect on both gnihr and kiss2r mRNA levels. To 
      the best of our knowledge, this is the first description of a direct and 
      differential regulation of reproduction-related gene expression by Kiss2 at the 
      hypothalamus level of a teleost fish. Overall, this study provides novel 
      information on the role of Kiss2/Kiss2R system in the reproductive function of 
      teleosts.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Wang, Bin
AU  - Wang B
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
FAU - Liu, Quan
AU  - Liu Q
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; College of Fisheries and Life Science, Shanghai 
      Ocean University, Shanghai 201306, China.
FAU - Liu, Xuezhou
AU  - Liu X
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China. Electronic address: liuxz@ysfri.ac.cn.
FAU - Xu, Yongjiang
AU  - Xu Y
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
FAU - Shi, Bao
AU  - Shi B
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
LA  - eng
PT  - Journal Article
DEP - 20170421
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (DNA, Complementary)
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Flatfishes/*genetics
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Hypothalamus/*metabolism
MH  - Kisspeptins/*genetics/metabolism
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cell Surface/chemistry/*genetics/metabolism
MH  - Reproduction/*genetics
MH  - Sequence Alignment
MH  - Sexual Maturation
OTO - NOTNLM
OT  - Cynoglossus semilaevis
OT  - Hypothalamus
OT  - Kisspeptin
OT  - Kisspeptin receptor
OT  - cDNA cloning
EDAT- 2017/04/26 06:00
MHDA- 2017/11/07 06:00
CRDT- 2017/04/26 06:00
PHST- 2016/12/26 00:00 [received]
PHST- 2017/03/12 00:00 [revised]
PHST- 2017/04/10 00:00 [accepted]
PHST- 2017/04/26 06:00 [pubmed]
PHST- 2017/11/07 06:00 [medline]
PHST- 2017/04/26 06:00 [entrez]
AID - S0016-6480(17)30264-2 [pii]
AID - 10.1016/j.ygcen.2017.04.006 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2017 Aug 1;249:55-63. doi: 10.1016/j.ygcen.2017.04.006. Epub 
      2017 Apr 21.