PMID- 28439930
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20220408
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 57
IP  - 7
DP  - 2017 Jul
TI  - Detection and identification of platelet antibodies using a sensitive multiplex 
      assay system-platelet antibody bead array.
PG  - 1724-1733
LID - 10.1111/trf.14122 [doi]
AB  - BACKGROUND: Tests for platelet-specific antibodies are important in the diagnosis 
      of immune platelet disorders. Monoclonal antibody glycoprotein capture assays 
      have been the gold standards in platelet antibody detection for almost 30 years. 
      However, such assays are complex and cumbersome to perform, which limits their 
      routine use. We report the performance of a newly developed, easy to perform 
      platelet antibody bead array (PABA) for the detection of platelet-specific 
      antibodies. STUDY DESIGN AND METHODS: PABA is the equivalent of the monoclonal 
      antigen capture enzyme-linked immunosorbent assay (ELISA) (MACE) on a bead and 
      instead with fluorescent detection of immunoglobulin (Ig)G platelet antibodies by 
      Luminex. Antibodies against platelet glycoproteins (GP) GPIIb/IIIa, GPIb/IX, 
      GPIa/IIa, GPIV, and class I human leukocyte antigen (HLA) could be detected in a 
      patient's serum simultaneously in a single well of a microplate. Results from 80 
      patient samples and 20 normal donor samples were compared using PABA, MACE, and a 
      commercial ELISA kit. RESULTS: PABA detected all antibodies, with specificity for 
      human platelet antigens (HPAs) HPA-1a, HPA-1b, HPA-2a, HPA-2b, HPA-3a, HPA-3b, 
      HPA-4a, HPA-4b, HPA-5a, HPA-5b, HPA-15b, and HLA. Overall, compared with MACE, 
      the sensitivity and specificity of PABA were 99% and 97%, respectively, and both 
      were significantly better than those of the commercial ELISA. PABA had greater 
      analytic sensitivity than MACE for HPA-1a, HPA-3a, and HPA-5b antibodies. In 
      addition, PABA detected HPA-5b and HPA-3b antibodies that were missed by MACE. 
      The overall false-positive rate of PABA compared with MACE was 2.7%. CONCLUSIONS: 
      The PABA is a rapid, highly sensitive and specific, multiplex bead-based assay 
      for detecting human platelet antibodies. Such a simple yet high-throughput 
      platform will facilitate practical, routine testing for the identification of 
      platelet-specific antibodies.
CI  - (c) 2017 AABB.
FAU - Metzner, Krista
AU  - Metzner K
AD  - Platelet and Neutrophil Immunology Laboratory, Milwaukee, Wisconsin.
FAU - Bauer, Julie
AU  - Bauer J
AD  - Information Services, Milwaukee, Wisconsin.
FAU - Ponzi, Heather
AU  - Ponzi H
AD  - Platelet and Neutrophil Immunology Laboratory, Milwaukee, Wisconsin.
FAU - Ujcich, Allison
AU  - Ujcich A
AD  - Platelet and Neutrophil Immunology Laboratory, Milwaukee, Wisconsin.
FAU - Curtis, Brian R
AU  - Curtis BR
AD  - Platelet and Neutrophil Immunology Laboratory, Milwaukee, Wisconsin.
AD  - Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin.
LA  - eng
PT  - Journal Article
DEP - 20170425
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Blood Platelets/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Isoantibodies/*blood
EDAT- 2017/04/26 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/04/26 06:00
PHST- 2016/11/23 00:00 [received]
PHST- 2017/02/10 00:00 [revised]
PHST- 2017/02/26 00:00 [accepted]
PHST- 2017/04/26 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/04/26 06:00 [entrez]
AID - 10.1111/trf.14122 [doi]
PST - ppublish
SO  - Transfusion. 2017 Jul;57(7):1724-1733. doi: 10.1111/trf.14122. Epub 2017 Apr 25.