PMID- 28440316
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR  - 20220330
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Apr 25
TI  - Modest and Severe Maternal Iron Deficiency in Pregnancy are Associated with Fetal 
      Anaemia and Organ-Specific Hypoxia in Rats.
PG  - 46573
LID - 10.1038/srep46573 [doi]
LID - 46573
AB  - Prenatal iron-deficiency (ID) is known to alter fetal developmental trajectories, 
      which predisposes the offspring to chronic disease in later life, although the 
      underlying mechanisms remain unclear. Here, we sought to determine whether 
      varying degrees of maternal anaemia could induce organ-specific patterns of 
      hypoxia in the fetuses. Pregnant female Sprague Dawley rats were fed 
      iron-restricted or iron-replete diets to induce a state of moderate (M-ID) or 
      severe ID (S-ID) alongside respective controls. Ultrasound biomicroscopy was 
      performed on gestational day (GD)20 to assess uterine and umbilical artery blood 
      flow patterns. On GD21, tissues were collected and assessed for hypoxia using 
      pimonidazole staining. Compared to controls, maternal haemoglobin (Hb) in M- and 
      S-ID were reduced 17% (P < 0.01) and 48% (P < 0.001), corresponding to 39% 
      (P < 0.001) and 65% (P < 0.001) decreases in fetal Hb. Prenatal ID caused 
      asymmetric fetal growth restriction, which was most pronounced in S-ID. In both 
      severities of ID, umbilical artery resistive index was increased (P < 0.01), 
      while pulsatility index only increased in S-ID (P < 0.05). In both M-and S-ID, 
      fetal kidneys and livers showed evidence of hypoxia (P < 0.01 vs. controls), 
      whereas fetal brains and placentae remained normoxic. These findings indicate 
      prenatal ID causes organ-specific fetal hypoxia, even in the absence of severe 
      maternal anaemia.
FAU - Woodman, Andrew G
AU  - Woodman AG
AD  - Department of Pharmacology, University of Alberta, Edmonton, Canada.
FAU - Care, Alison S
AU  - Care AS
AD  - Department of Obstetrics and Gynaecology, University of Alberta, Edmonton, 
      Canada.
FAU - Mansour, Yael
AU  - Mansour Y
AD  - Department of Anesthesiology &Pain Medicine, University of Alberta, Edmonton, 
      Canada.
FAU - Cherak, Stephana J
AU  - Cherak SJ
AD  - Department of Pharmacology, University of Alberta, Edmonton, Canada.
FAU - Panahi, Sareh
AU  - Panahi S
AD  - Department of Anesthesiology &Pain Medicine, University of Alberta, Edmonton, 
      Canada.
FAU - Gragasin, Ferrante S
AU  - Gragasin FS
AD  - Department of Anesthesiology &Pain Medicine, University of Alberta, Edmonton, 
      Canada.
FAU - Bourque, Stephane L
AU  - Bourque SL
AD  - Department of Pharmacology, University of Alberta, Edmonton, Canada.
AD  - Department of Anesthesiology &Pain Medicine, University of Alberta, Edmonton, 
      Canada.
LA  - eng
GR  - MOP142396/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170425
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - *Anemia, Iron-Deficiency/blood/embryology/pathology
MH  - Animals
MH  - *Brain/blood supply/embryology/pathology
MH  - Female
MH  - Fetal Diseases/*blood
MH  - *Iron Deficiencies
MH  - *Placenta/blood supply/embryology/pathology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC5404227
COIS- The authors declare no competing financial interests.
EDAT- 2017/04/26 06:00
MHDA- 2019/07/16 06:00
PMCR- 2017/04/25
CRDT- 2017/04/26 06:00
PHST- 2016/12/01 00:00 [received]
PHST- 2017/03/17 00:00 [accepted]
PHST- 2017/04/26 06:00 [entrez]
PHST- 2017/04/26 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
PHST- 2017/04/25 00:00 [pmc-release]
AID - srep46573 [pii]
AID - 10.1038/srep46573 [doi]
PST - epublish
SO  - Sci Rep. 2017 Apr 25;7:46573. doi: 10.1038/srep46573.