PMID- 28441774
OWN - NLM
STAT- MEDLINE
DCOM- 20180119
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Apr 24
TI  - Brain-Derived Neurotrophic Factor Mediated Perfluorooctane Sulfonate 
      Induced-Neurotoxicity via Epigenetics Regulation in SK-N-SH Cells.
LID - 10.3390/ijms18040893 [doi]
LID - 893
AB  - Perfluorooctane sulfonate (PFOS), a new kind of persistent organic pollutant, is 
      widely distributed in the environment and exists in various organisms, where it 
      is also a neurotoxic compound. However, the potential mechanism of its 
      neurotoxicity is still unclear. To examine the role of epigenetics in the 
      neurotoxicity induced by PFOS, SK-N-SH cells were treated with different 
      concentrations of PFOS or control medium (0.1% DMSO) for 48 h. The mRNA levels of 
      DNA methyltransferases (DNMTs) and Brain-derived neurotrophic factor (BDNF), 
      microRNA-16, microRNA-22, and microRNA-30a-5p were detected by Quantitative PCR 
      (QPCR). Enzyme Linked Immunosorbent Assay (ELISA) was used to measure the protein 
      levels of BDNF, and a western blot was applied to analyze the protein levels of 
      DNMTs. Bisulfite sequencing PCR (BSP) was used to detect the methylation status 
      of the BDNF promoter I and IV. Results of MTT assays indicated that treatment 
      with PFOS could lead to a significant decrease of cell viability, and the treated 
      cells became shrunk. In addition, PFOS exposure decreased the expression of BDNF 
      at mRNA and protein levels, increased the expression of microRNA-16, microRNA-22, 
      microRNA-30a-5p, and decreased the expression of DNMT1 at mRNA and protein 
      levels, but increased the expression of DNMT3b at mRNA and protein levels. Our 
      results also demonstrate that PFOS exposure changes the methylation status of 
      BDNF promoter I and IV. The findings of the present study suggest that 
      methylation regulation of BDNF gene promoter and increases of 
      BDNF-related-microRNA might underlie the mechanisms of PFOS-induced 
      neurotoxicity.
FAU - Guo, Xin-Xin
AU  - Guo XX
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. guoxinxin1677@gmail.com.
FAU - He, Qing-Zhi
AU  - He QZ
AD  - School of Pharmacy and Biology, University of South China, Hengyang 421001, 
      China. heqingzhi666@gmail.com.
AD  - Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 
      Hengyang 421001, China. heqingzhi666@gmail.com.
FAU - Li, Wu
AU  - Li W
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. liwu199103@gmail.com.
FAU - Long, Ding-Xin
AU  - Long DX
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. longdx@usc.edu.cn.
FAU - Pan, Xiao-Yuan
AU  - Pan XY
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. panxiaoyuan199104@gmail.com.
FAU - Chen, Cong
AU  - Chen C
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. chencong199206@gmail.com.
FAU - Zeng, Huai-Cai
AU  - Zeng HC
AD  - Department of Preventive Medicine, School of Public Health, University of South 
      China, Hengyang 421001, China. zenghuaicai@usc.edu.cn.
AD  - Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, 
      Hengyang 421001, China. zenghuaicai@usc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170424
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 9H2MAI21CL (perfluorooctane sulfonic acid)
RN  - EC 2.1.1.- (DNA-Cytosine Methylases)
SB  - IM
MH  - Alkanesulfonic Acids/*toxicity
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - DNA Methylation/drug effects
MH  - DNA-Cytosine Methylases/genetics/metabolism
MH  - Environmental Pollutants/*toxicity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epigenesis, Genetic/*drug effects
MH  - Fluorocarbons/*toxicity
MH  - Gene Expression/drug effects
MH  - Humans
MH  - MicroRNAs/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC5412472
OTO - NOTNLM
OT  - BDNF
OT  - DNA methylation
OT  - DNMTs
OT  - PFOS
OT  - microRNA
EDAT- 2017/04/27 06:00
MHDA- 2018/01/20 06:00
PMCR- 2017/04/01
CRDT- 2017/04/27 06:00
PHST- 2017/02/20 00:00 [received]
PHST- 2017/04/17 00:00 [revised]
PHST- 2017/04/19 00:00 [accepted]
PHST- 2017/04/27 06:00 [entrez]
PHST- 2017/04/27 06:00 [pubmed]
PHST- 2018/01/20 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijms18040893 [pii]
AID - ijms-18-00893 [pii]
AID - 10.3390/ijms18040893 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Apr 24;18(4):893. doi: 10.3390/ijms18040893.