PMID- 28442288
OWN - NLM
STAT- MEDLINE
DCOM- 20180215
LR  - 20180215
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 78
IP  - 5-6
DP  - 2017 May-Jun
TI  - Expression and differential regulation of HLA-G isoforms in the retinal pigment 
      epithelial cell line, ARPE-19.
PG  - 414-420
LID - S0198-8859(17)30069-1 [pii]
LID - 10.1016/j.humimm.2017.04.007 [doi]
AB  - The purpose of this study was to examine if HLA-G is expressed in the retinal 
      pigment epithelium (RPE) cells of the eye. The RPE comprises the outer most layer 
      of the retina and as such defines the interface to the blood and contributes to 
      the immune privilege in the posterior part of the eye. One way the RPE might be 
      regulating the immune system could be by expressing the non-classical human 
      leukocyte antigen (HLA) molecule, HLA-G. We therefore sought to define if the RPE 
      cell line, ARPE-19, expressed HLA-G and analyse the regulation as a response to 
      pro-inflammatory cytokines. This was done by digital droplet PCR, measuring the 
      gene expression of HLA-G in total RNA. The protein expression was analysed by 
      immunohistochemistry and by immunofluorescence followed by confocal microscopy 
      and the expression of the HLA-G isoforms was explored by fragment analysis. In 
      the current study, we show that HLA-G is expressed by ARPE-19 cells and is 
      upregulated as a response to pro-inflammatory cytokines. Moreover, we are the 
      first to describe a differential regulation of the HLA-G isoforms as a direct 
      response to stimulation. These results might indicate that HLA-G can be part of 
      the immune privilege of the posterior part of the eye, but further experiments on 
      primary RPE cells are needed.
CI  - Copyright (c) 2017. Published by Elsevier Inc.
FAU - Svendsen, Signe Goul
AU  - Svendsen SG
AD  - Department of Immunology and Microbiology, University of Copenhagen, Denmark. 
      Electronic address: signe_svendsen@hotmail.com.
FAU - Udsen, Maja Soberg
AU  - Udsen MS
AD  - Department of Immunology and Microbiology, University of Copenhagen, Denmark.
FAU - Daouya, Marina
AU  - Daouya M
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, Paris, France.
FAU - Funck, Tina
AU  - Funck T
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
FAU - Wu, Ching-Lien
AU  - Wu CL
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, Paris, France.
FAU - Carosella, Edgardo D
AU  - Carosella ED
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, Paris, France.
FAU - LeMaoult, Joel
AU  - LeMaoult J
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, Paris, France.
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TVF
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
FAU - Faber, Carsten
AU  - Faber C
AD  - Department of Immunology and Microbiology, University of Copenhagen, Denmark; 
      Department of Ophthalmology, Rigshospitalet, Glostrup, Denmark.
FAU - Nissen, Mogens Holst
AU  - Nissen MH
AD  - Department of Immunology and Microbiology, University of Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20170422
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Cytokines)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Cell Line
MH  - Cytokines/immunology
MH  - Fluorescent Antibody Technique
MH  - Gene Expression
MH  - HLA-G Antigens/genetics/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation/*immunology
MH  - Inflammation Mediators/immunology
MH  - Microscopy, Confocal
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms/genetics/*metabolism
MH  - Retinal Pigment Epithelium/pathology/*physiology
OTO - NOTNLM
OT  - ARPE-19
OT  - HLA-G
OT  - Isoforms
OT  - Retinal pigment epithelium
OT  - sHLA-G
EDAT- 2017/04/27 06:00
MHDA- 2018/02/16 06:00
CRDT- 2017/04/27 06:00
PHST- 2016/11/30 00:00 [received]
PHST- 2017/04/20 00:00 [revised]
PHST- 2017/04/20 00:00 [accepted]
PHST- 2017/04/27 06:00 [pubmed]
PHST- 2018/02/16 06:00 [medline]
PHST- 2017/04/27 06:00 [entrez]
AID - S0198-8859(17)30069-1 [pii]
AID - 10.1016/j.humimm.2017.04.007 [doi]
PST - ppublish
SO  - Hum Immunol. 2017 May-Jun;78(5-6):414-420. doi: 10.1016/j.humimm.2017.04.007. 
      Epub 2017 Apr 22.