PMID- 28442601
OWN - NLM
STAT- MEDLINE
DCOM- 20180227
LR  - 20190610
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 37
IP  - 3
DP  - 2017 Jun 30
TI  - Crystal structure of thermostable alkylsulfatase SdsAP from Pseudomonas sp. S9.
LID - BSR20170001 [pii]
LID - 10.1042/BSR20170001 [doi]
AB  - A novel alkylsulfatase from bacterium Pseudomonas sp. S9 (SdsAP) was identified 
      as a thermostable alkylsulfatases (type III), which could hydrolyze the primary 
      alkyl sulfate such as sodium dodecyl sulfate (SDS). Thus, it has a potential 
      application of SDS biodegradation. The crystal structure of SdsAP has been solved 
      to a resolution of 1.76 A and reveals that SdsAP contains the characteristic 
      metallo-beta-lactamase-like fold domain, dimerization domain, and C-terminal sterol 
      carrier protein type 2 (SCP-2)-like fold domain. Kinetic characterization of 
      SdsAP to SDS by isothermal titration calorimetry (ITC) and enzymatic activity 
      assays of constructed mutants demonstrate that Y246 and G263 are important 
      residues for its preference for the hydrolysis of 'primary alkyl' chains, 
      confirming that SdsAP is a primary alkylsulfatase.
CI  - (c) 2017 The Author(s).
FAU - Sun, Lifang
AU  - Sun L
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
FAU - Chen, Pu
AU  - Chen P
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
FAU - Su, Yintao
AU  - Su Y
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
FAU - Cai, Zhixiong
AU  - Cai Z
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
FAU - Ruan, Lingwei
AU  - Ruan L
AD  - Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, 
      State Oceanic Administration (SOA), No. 178 Daxue Road, Xiamen 361005, China.
FAU - Xu, Xun
AU  - Xu X
AD  - Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, 
      State Oceanic Administration (SOA), No. 178 Daxue Road, Xiamen 361005, China.
FAU - Wu, Yunkun
AU  - Wu Y
AD  - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the 
      Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China 
      wuyk@fjirsm.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Bacterial Proteins)
RN  - 0 (Sulfates)
RN  - 0 (Surface-Active Agents)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - EC 3.1.6.- (Sulfatases)
RN  - EC 3.1.6.- (alkylsulfatase)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Bacterial Proteins/*chemistry
MH  - Hydrolysis
MH  - Kinetics
MH  - Pseudomonas/*metabolism
MH  - Sodium Dodecyl Sulfate/chemistry
MH  - Substrate Specificity
MH  - Sulfatases/*chemistry
MH  - Sulfates/chemistry
MH  - Surface-Active Agents/chemistry
MH  - beta-Lactamases/chemistry
PMC - PMC5426288
OTO - NOTNLM
OT  - Pseudomonas sp.
OT  - SCP-2-like fold domain
OT  - SDS
OT  - SdsAP
OT  - alkylsulfatase
OT  - crystal structure
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2017/04/27 06:00
MHDA- 2018/02/28 06:00
PMCR- 2017/05/11
CRDT- 2017/04/27 06:00
PHST- 2017/01/01 00:00 [received]
PHST- 2017/04/24 00:00 [revised]
PHST- 2017/04/25 00:00 [accepted]
PHST- 2017/04/27 06:00 [pubmed]
PHST- 2018/02/28 06:00 [medline]
PHST- 2017/04/27 06:00 [entrez]
PHST- 2017/05/11 00:00 [pmc-release]
AID - BSR20170001 [pii]
AID - 10.1042/BSR20170001 [doi]
PST - epublish
SO  - Biosci Rep. 2017 May 11;37(3):BSR20170001. doi: 10.1042/BSR20170001. Print 2017 
      Jun 30.