PMID- 28445603 OWN - NLM STAT- MEDLINE DCOM- 20180615 LR - 20180615 IS - 1520-6033 (Electronic) IS - 1520-6033 (Linking) VI - 33 IP - 4 DP - 2017 Jul TI - Alternating flow filtration as an alternative to internal spin filter based perfusion process: Impact on productivity and product quality. PG - 1010-1014 LID - 10.1002/btpr.2487 [doi] AB - This article reports the results obtained from comparison of internal spin filter (ISF) and alternating flow filtration (ATF) as cell retention systems, regarding cell growth, volumetric perfusion rate, cell specific perfusion rate and cell productivity in the fermentation process. As expected we were able to reach higher cell densities and to achieve longer runs since ATF systems are known to be less affected by fouling. Volumetric production of the reactor using the ATF system was 50-70% higher than the production achieved using the ISF due to higher cell density and a two-fold increase in the perfusion rate. On the other hand, downstream processing performances were evaluated regarding chromatographic steps yields and productivity and quality attributes of the purified materials. Similar results were obtained for all evaluated systems. The fact that we were able to achieve a 2 working volumes (WV)/day perfusion rate using an ATF system as cell retention device allowed us to virtually double the WV of a 25 L reactor. These results constitute valuable data for the optimization of recombinant protein production in perfusion processes since a two-fold increase in the average production of a manufacturing facility could be easily achieved as long as downstream scale up is possible. (c) 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1010-1014, 2017. CI - (c) 2017 American Institute of Chemical Engineers. FAU - Bosco, Belen AU - Bosco B AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. FAU - Paillet, Cristian AU - Paillet C AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. FAU - Amadeo, Ignacio AU - Amadeo I AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. AD - Laboratorio de Cultivos Celulares, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, (3000) Santa Fe, Argentina. FAU - Mauro, Laura AU - Mauro L AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. FAU - Orti, Eduardo AU - Orti E AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. FAU - Forno, Guillermina AU - Forno G AD - Zelltek S.A. RN 168, Parque Tecnologico Litoral Centro, (3000) Santa Fe, Argentina. AD - Laboratorio de Cultivos Celulares, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, (3000) Santa Fe, Argentina. LA - eng PT - Journal Article DEP - 20170514 PL - United States TA - Biotechnol Prog JT - Biotechnology progress JID - 8506292 RN - 0 (Recombinant Proteins) SB - IM MH - Animals MH - *Bioreactors MH - CHO Cells MH - Cell Count MH - *Cell Culture Techniques MH - Cell Survival MH - Cells, Cultured MH - Cricetulus MH - *Filtration MH - *Perfusion MH - Recombinant Proteins/biosynthesis/isolation & purification OTO - NOTNLM OT - Alternate Tangential Perfusion OT - CHO OT - Cell specific perfusion feed rates OT - Optimization EDAT- 2017/04/27 06:00 MHDA- 2018/06/16 06:00 CRDT- 2017/04/27 06:00 PHST- 2016/08/31 00:00 [received] PHST- 2017/03/13 00:00 [revised] PHST- 2017/04/27 06:00 [pubmed] PHST- 2018/06/16 06:00 [medline] PHST- 2017/04/27 06:00 [entrez] AID - 10.1002/btpr.2487 [doi] PST - ppublish SO - Biotechnol Prog. 2017 Jul;33(4):1010-1014. doi: 10.1002/btpr.2487. Epub 2017 May 14.