PMID- 28445813 OWN - NLM STAT- MEDLINE DCOM- 20180214 LR - 20180823 IS - 1873-4235 (Electronic) IS - 0956-5663 (Linking) VI - 95 DP - 2017 Sep 15 TI - Microfluidic-integrated patterned ITO immunosensor for rapid detection of prostate-specific membrane antigen biomarker in prostate cancer. PG - 160-167 LID - S0956-5663(17)30245-2 [pii] LID - 10.1016/j.bios.2017.04.004 [doi] AB - An optically transparent patterned indium tin oxide (ITO) three-electrode sensor integrated with a microfluidic channel was designed for label-free immunosensing of prostate-specific membrane antigen (PSMA), a prostate cancer (PCa) biomarker, expressed on prostate tissue and circulating tumor cells but also found in serum. The sensor relies on cysteamine capped gold nanoparticles (N-AuNPs) covalently linked with anti-PSMA antibody (Ab) for target specificity. A polydimethylsiloxane (PDMS) microfluidic channel is used to efficiently and reproducibly introduce sample containing soluble proteins/cells to the sensor. The PSMA is detected and quantified by measuring the change in differential pulse voltammetry signal of a redox probe ([Fe(CN)(6)](3-)/[Fe(CN)(6)](4-)) that is altered upon binding of PSMA with PSMA-Ab immobilized on N-AuNPs/ITO. Detection of PSMA expressing cells and soluble PSMA was tested. The limit of detection (LOD) of the sensor for PSMA-based PCa cells is 6/40microL (i.e., 150 cells/mL) (n=3) with a linear range of 15-400 cells/40microL (i.e., 375-10,000 cells/mL), and for the soluble PSMA is 0.499ng/40microL (i.e., 12.5ng/mL) (n=3) with the linear range of 0.75-250ng/40microL (i.e., 19-6250ng/mL), both with an incubation time of 10min. The results indicate that the sensor has a suitable sensitivity and dynamic range for routine detection of PCa circulating tumor cells and can be adapted to detect other biomarkers/cancer cells. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Seenivasan, Rajesh AU - Seenivasan R AD - Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 H Mall, Madison, WI 53706, USA. Electronic address: seenivasan@wisc.edu. FAU - Singh, Chandra K AU - Singh CK AD - Department of Dermatology, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA. Electronic address: cksingh@wisc.edu. FAU - Warrick, Jay W AU - Warrick JW AD - Department of Biomedical Engineering, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI, 53705, USA. Electronic address: warrick@wisc.edu. FAU - Ahmad, Nihal AU - Ahmad N AD - Department of Dermatology, University of Wisconsin-Madison, 1300 University Avenue, Madison, WI 53706, USA. Electronic address: nahmad@wisc.edu. FAU - Gunasekaran, Sundaram AU - Gunasekaran S AD - Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 H Mall, Madison, WI 53706, USA. Electronic address: guna@wisc.edu. LA - eng PT - Journal Article DEP - 20170411 PL - England TA - Biosens Bioelectron JT - Biosensors & bioelectronics JID - 9001289 RN - 0 (Antigens, Surface) RN - 0 (Biomarkers, Tumor) RN - EC 3.4.17.21 (FOLH1 protein, human) RN - EC 3.4.17.21 (Glutamate Carboxypeptidase II) SB - IM MH - Antigens, Surface/*blood MH - Biomarkers, Tumor/*blood MH - *Biosensing Techniques MH - Glutamate Carboxypeptidase II/*blood MH - Humans MH - Male MH - Metal Nanoparticles MH - Microfluidics MH - Neoplastic Cells, Circulating MH - Prostatic Neoplasms/*blood OTO - NOTNLM OT - Electrostatic OT - ITO three-electrode patterning OT - Immunocomplex OT - Microfluidic OT - PSMA OT - Prostate cancer EDAT- 2017/04/27 06:00 MHDA- 2018/02/15 06:00 CRDT- 2017/04/27 06:00 PHST- 2017/01/23 00:00 [received] PHST- 2017/03/30 00:00 [revised] PHST- 2017/04/10 00:00 [accepted] PHST- 2017/04/27 06:00 [pubmed] PHST- 2018/02/15 06:00 [medline] PHST- 2017/04/27 06:00 [entrez] AID - S0956-5663(17)30245-2 [pii] AID - 10.1016/j.bios.2017.04.004 [doi] PST - ppublish SO - Biosens Bioelectron. 2017 Sep 15;95:160-167. doi: 10.1016/j.bios.2017.04.004. Epub 2017 Apr 11.