PMID- 28446413 OWN - NLM STAT- MEDLINE DCOM- 20171019 LR - 20200612 IS - 1673-4254 (Print) IS - 2663-0842 (Electronic) IS - 1673-4254 (Linking) VI - 37 IP - 4 DP - 2016 Apr 20 TI - [Effects of recombinant human erythropoietin on brain-derived neurotrophic factor expression in different brain regions of aging rats]. PG - 551-554 AB - OBJECTIVE: To explore the effect of recombinant human erythropoietin (rhEPO) on expression of brain-derived neurotrophic factor (BDNF) in different brain regions of aging rats. METHODS: Forty male SD rats were randomized equally into negative control group, D-galactose group, EPO treatment group, and positive control group. Rat models of subacute aging were established by continuous subcutaneous injection of 5% D-galactose. Immunohistochemical staining was used to analyze the variation of BDNF expressions in different brain regions of the aging rats with different treatments. RESULTS: Significant brain region-specific differences in BDNF expression were found among the rats in different groups. Compared with those in the negative control group, the numbers of BDNF-positive cells in the hippocampal CA1 region, CA3 region, dentate gyrus (DG) and frontal cortex were all decreased obviously in D-galactose group (P<0.05) but increased in both EPO group and the positive control group (P<0.05) without significant differences between the latter two groups. In the rats in the same group, the number of BDNF-positive cells varied markedly in different brain regions (P<0.05), and the expression level of BDNF was the highest in the frontal cortex followed by the hippocampal CA3 region and the dentate gyrus, and was the lowest in the hippocampal CA1 region. CONCLUSION: Treatment with rhEPO enhances the expression of BDNF in rat neural cells, suggesting that rhEPO may protect the nervous system from aging by regulating the BDNF pathway. FAU - Wang, Hu-Qing AU - Wang HQ AD - Department of Neurology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. E-mail: tigerwhq@sohu.com. FAU - Gao, Zhen AU - Gao Z FAU - Chen, Meng-Yi AU - Chen MY FAU - Wu, Hai-Qin AU - Wu HQ FAU - Zhang, Gui-Lian AU - Zhang GL FAU - Zhan, Shu-Qin AU - Zhan SQ FAU - Bu, Ning AU - Bu N FAU - Liu, Jing-Jie AU - Liu JJ FAU - Zhai, Yue-Fen AU - Zhai YF LA - chi PT - Journal Article PL - China TA - Nan Fang Yi Ke Da Xue Xue Bao JT - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JID - 101266132 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Recombinant Proteins) RN - 11096-26-7 (Erythropoietin) RN - X2RN3Q8DNE (Galactose) SB - IM MH - *Aging MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - CA1 Region, Hippocampal/metabolism MH - CA3 Region, Hippocampal/metabolism MH - Dentate Gyrus/metabolism MH - Erythropoietin/*pharmacology MH - Frontal Lobe/metabolism MH - Galactose MH - Humans MH - Male MH - Neurons/*drug effects/metabolism MH - Random Allocation MH - Rats MH - Rats, Sprague-Dawley MH - Recombinant Proteins/pharmacology PMC - PMC6744085 EDAT- 2017/04/28 06:00 MHDA- 2017/10/20 06:00 PMCR- 2017/04/20 CRDT- 2017/04/28 06:00 PHST- 2017/04/28 06:00 [entrez] PHST- 2017/04/28 06:00 [pubmed] PHST- 2017/10/20 06:00 [medline] PHST- 2017/04/20 00:00 [pmc-release] AID - nfykdxxb-37-4-551 [pii] AID - 10.3969/j.issn.1673-4254.2017.04.23 [doi] PST - ppublish SO - Nan Fang Yi Ke Da Xue Xue Bao. 2016 Apr 20;37(4):551-554. doi: 10.3969/j.issn.1673-4254.2017.04.23.