PMID- 28447757
OWN - NLM
STAT- MEDLINE
DCOM- 20180220
LR  - 20210109
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 15
IP  - 5
DP  - 2017 May
TI  - Molecular mechanism of smurf2 in regulating the expression of SnoN in diabetic 
      nephropathy.
PG  - 2560-2566
LID - 10.3892/mmr.2017.6307 [doi]
AB  - The aim of the present study was to examine the regulatory mechanism underlying 
      the depression in Ski‑related novel protein N (SnoN) in diabetic nephrology (DN). 
      NRK‑52E cells, a rat primary renal tubular epithelial cell line, were cultured to 
      clarify the effect of small mothers against decapentaplegic (Smad) ubiquitination 
      regulatory factor 2 (smurf2) on SnoN in a low glucose environment in vitro. 
      NRK‑52E cells and DM rats were injected with adenoviruses AD‑smurf2 and 
      AD‑shsmurf2, respectively, and the protein expression profiles of SnoN, smurf2 
      and phosphorylated (p)‑Smad2 were then detected. In addition, the protein levels 
      of smurf2, p‑Smad2 and SnoN were analyzed following treatment with transforming 
      growth factor (TGF)‑beta1 or TGF‑beta1 inhibitor to validate the effect of the 
      TGF‑beta1/Smad signaling pathway. The effect of smurf2 on the degradation of SnoN by 
      ubiquitination was found to be a key factor in DN, which was mediated by the 
      TGF‑beta1/Smad signaling pathway.
FAU - Xu, Zhuojia
AU  - Xu Z
AD  - Department of Nephrology, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, P.R. China.
FAU - Diao, Zongli
AU  - Diao Z
AD  - Department of Nephrology, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, P.R. China.
FAU - Liu, Ruixia
AU  - Liu R
AD  - Department of Infectious Disease, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, P.R. China.
FAU - Liu, Wenhu
AU  - Liu W
AD  - Department of Nephrology, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Skil_v1 protein, rat)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad2 protein, rat)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 2.3.2.26 (Smurf2 protein, rat)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/genetics/*metabolism/pathology
MH  - Diabetic Nephropathies/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Male
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - Rats, Sprague-Dawley
MH  - *Signal Transduction
MH  - Smad2 Protein/genetics/metabolism
MH  - Transcription Factors/*biosynthesis/genetics
MH  - Transforming Growth Factor beta1/genetics/metabolism
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
PMC - PMC5428923
EDAT- 2017/04/28 06:00
MHDA- 2018/02/21 06:00
PMCR- 2017/03/09
CRDT- 2017/04/28 06:00
PHST- 2016/01/22 00:00 [received]
PHST- 2017/01/12 00:00 [accepted]
PHST- 2017/04/28 06:00 [pubmed]
PHST- 2018/02/21 06:00 [medline]
PHST- 2017/04/28 06:00 [entrez]
PHST- 2017/03/09 00:00 [pmc-release]
AID - mmr-15-05-2560 [pii]
AID - 10.3892/mmr.2017.6307 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 May;15(5):2560-2566. doi: 10.3892/mmr.2017.6307. Epub 2017 Mar 
      9.