PMID- 28448991
OWN - NLM
STAT- MEDLINE
DCOM- 20170711
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 41
IP  - 6
DP  - 2017
TI  - Participation of Antidiuretic Hormone (ADH) in Asthma Exacerbations Induced by 
      Psychological Stress via PKA/PKC Signal Pathway in Airway-Related Vagal 
      Preganglionic Neurons (AVPNs).
PG  - 2230-2241
LID - 10.1159/000475638 [doi]
AB  - AIMS: Present study was performed to examine whether ADH was implicated in 
      psychological stress asthma and to explore the underlying molecular mechanism. 
      METHODS: We not only examined ADH levels in the cerebrospinal fluid (CSF) via 
      radioimmunoassay, but also measured ADH receptor (ADHR) expression in 
      airway-related vagal preganglionic neurons (AVPNs) through real-time PCR in all 
      experimental mice. Western blotting was performed to evaluate the relationship 
      between ADH and PKA/PKC in psychological stress asthma. Finally, the role of 
      PKA/PKC in psychological stress asthma was analyzed. RESULTS: Marked asthma 
      exacerbations were noted owing to significantly elevated levels of ADH and ADHR 
      after psychological stress induction as compared to OVA alone (asthma group). 
      ADHR antagonists (SR-49095 or SR-121463A) dramatically lowered higher protein 
      levels of PKAalpha and PKCalpha induced by psychological stress as compared to OVA alone, 
      suggesting the correlation between ADH and PKA/PKC in psychological stress 
      asthma. KT-5720 (PKA inhibitor) and Go-7874 (PKC inhibitor) further directly 
      revealed the involvement of PKA/PKC in psychological stress asthma. Some notable 
      changes were also noted after employing PKA and PKC inhibitors in psychological 
      stress asthma, including reduced asthmatic inflammation (lower eosinophil 
      peroxidase (EPO) activity, myeloperoxidase (MPO) activity, immunoglobulin E (IgE) 
      level, and histamine release), substantial decrements in inflammatory cell counts 
      (eosinophils and lymphocytes), and decreased cytokine secretion (IL-6, IL-10, and 
      IFN-gamma), indicating the involvement of PKA/PKC in asthma exacerbations induced by 
      psychological stress. CONCLUSION: Our results strongly suggested that ADH 
      participated in psychological stress-induced asthma exacerbations via PKA/PKC 
      signal pathway in AVPNs.
CI  - (c) 2017 The Author(s)Published by S. Karger AG, Basel.
FAU - Hou, Lili
AU  - Hou L
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Zhu, Lei
AU  - Zhu L
AD  - Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, 
      Shanghai, China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Zhang, Xingyi
AU  - Zhang X
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Zhang, Guoqing
AU  - Zhang G
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Liu, Zhenwei
AU  - Liu Z
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Li, Qiang
AU  - Li Q
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai 
      Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20170425
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antidiuretic Hormone Receptor Antagonists)
RN  - 0 (Carbazoles)
RN  - 0 (Cytokines)
RN  - 0 (Morpholines)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Pyrroles)
RN  - 0 (Receptors, Vasopressin)
RN  - 0 (Spiro Compounds)
RN  - 11000-17-2 (Vasopressins)
RN  - 58HV29I28S (KT 5720)
RN  - 9006-59-1 (Ovalbumin)
RN  - AJS8S3P31H (satavaptan)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Antidiuretic Hormone Receptor Antagonists/pharmacology
MH  - Asthma/etiology/metabolism
MH  - Carbazoles/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Eosinophils/cytology/metabolism
MH  - Female
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Morpholines/pharmacology
MH  - Neurons/*metabolism
MH  - Ovalbumin/immunology
MH  - Protein Kinase C/antagonists & inhibitors/*metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Pyrroles/pharmacology
MH  - Receptors, Vasopressin/chemistry/metabolism
MH  - Signal Transduction/drug effects
MH  - Spiro Compounds/pharmacology
MH  - *Stress, Psychological
MH  - Vasopressins/cerebrospinal fluid/*metabolism
OTO - NOTNLM
OT  - Antidiuretic hormone (ADH)
OT  - Asthma
OT  - PKA/PKC
OT  - Psychological stress
EDAT- 2017/04/28 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/04/28 06:00
PHST- 2016/07/14 00:00 [received]
PHST- 2016/11/11 00:00 [accepted]
PHST- 2017/04/28 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2017/04/28 06:00 [entrez]
AID - 000475638 [pii]
AID - 10.1159/000475638 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2017;41(6):2230-2241. doi: 10.1159/000475638. Epub 2017 Apr 
      25.