PMID- 28449125
OWN - NLM
STAT- MEDLINE
DCOM- 20180410
LR  - 20181202
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 93
IP  - 5
DP  - 2017 May 1
TI  - Saccharomyces cerevisiae accumulates GAPDH-derived peptides on its cell surface 
      that induce death of non-Saccharomyces yeasts by cell-to-cell contact.
LID - 10.1093/femsec/fix055 [doi]
AB  - During wine fermentations, Saccharomyces cerevisiae starts to excrete 
      antimicrobial peptides (AMPs) into the growth medium that induce death of 
      non-Saccharomyces yeasts at the end of exponential growth phase (24-48 h). Those 
      AMPs were found to derive from the glycolytic enzyme glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH). On the other hand, the early death of non-Saccharomyces 
      yeasts during wine fermentations was also found to be mediated by a cell-to-cell 
      contact mechanism. Since GAPDH is a cell-wall-associated protein in S. 
      cerevisiae, we put forward the hypothesis that the GAPDH-derived AMPs could 
      accumulate on the cell surface of S. cerevisiae, thus inducing death of 
      non-Saccharomyces yeasts by cell-to-cell contact. Here we show that 48-h grown 
      (stationary phase) cells of S. cerevisiae induce death of Hanseniaspora 
      guilliermondii and Lachancea thermotolerans by direct cell-to-cell contact, while 
      12-h grown cells (mid-exponential phase) do not. Immunological tests performed 
      with a specific polyclonal antibody against the GAPDH-derived AMPs revealed their 
      presence in the cell wall of S. cerevisiae cells grown for 48 h, but not for 12 
      h. Taken together, our data show that accumulation of GAPDH-derived AMPs on the 
      cell surface of S. cerevisiae is one of the factors underlying death of 
      non-Saccharomyces yeasts by cell-to-cell contact.
CI  - (c) FEMS 2017. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Branco, Patricia
AU  - Branco P
AD  - Unit of Bioenergy, Laboratorio Nacional de Energia e Geologia (LNEG), Estrada do 
      Paco do Lumiar 22, 1649-038 Lisboa, Portugal.
AD  - LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de 
      Agronomia, Universidade de Lisboa, Tapada da Ajuda 1349-017 Lisboa, Portugal.
FAU - Kemsawasd, Varongsiri
AU  - Kemsawasd V
AD  - Department of Food Science, Faculty of Science, University of Copenhagen, 
      Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
FAU - Santos, Lara
AU  - Santos L
AD  - Instituto Superior de Saude Egas Moniz, Campus Universitario, Quinta da Granja, 
      2829-511 Monte Caparica, Portugal.
FAU - Diniz, Mario
AU  - Diniz M
AD  - Instituto Superior de Saude Egas Moniz, Campus Universitario, Quinta da Granja, 
      2829-511 Monte Caparica, Portugal.
FAU - Caldeira, Jorge
AU  - Caldeira J
AD  - Instituto Superior de Saude Egas Moniz, Campus Universitario, Quinta da Granja, 
      2829-511 Monte Caparica, Portugal.
AD  - REQUIMTE- Dept. Quimica, Faculdade de Ciencias e Tecnologia (UNL), 2829-516 Monte 
      Caparica, Portugal.
FAU - Almeida, Maria Gabriela
AU  - Almeida MG
AD  - Instituto Superior de Saude Egas Moniz, Campus Universitario, Quinta da Granja, 
      2829-511 Monte Caparica, Portugal.
AD  - REQUIMTE- Dept. Quimica, Faculdade de Ciencias e Tecnologia (UNL), 2829-516 Monte 
      Caparica, Portugal.
FAU - Arneborg, Nils
AU  - Arneborg N
AD  - Department of Food Science, Faculty of Science, University of Copenhagen, 
      Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
FAU - Albergaria, Helena
AU  - Albergaria H
AD  - Unit of Bioenergy, Laboratorio Nacional de Energia e Geologia (LNEG), Estrada do 
      Paco do Lumiar 22, 1649-038 Lisboa, Portugal.
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Antimicrobial Cationic Peptides/*metabolism
MH  - Cell Membrane/metabolism
MH  - Fermentation
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Hanseniaspora/*metabolism
MH  - Microbial Interactions/*physiology
MH  - Saccharomyces cerevisiae/*enzymology/metabolism
MH  - Saccharomycetales/*metabolism
MH  - Wine/microbiology
OTO - NOTNLM
OT  - antimicrobial peptides
OT  - cell surface proteins
OT  - ecological dominance
OT  - glyceraldehyde-3-phosphate dehydrogenase
OT  - microbial interactions
OT  - wine fermentation
EDAT- 2017/04/28 06:00
MHDA- 2018/04/11 06:00
CRDT- 2017/04/28 06:00
PHST- 2017/02/06 00:00 [received]
PHST- 2017/04/21 00:00 [accepted]
PHST- 2017/04/28 06:00 [pubmed]
PHST- 2018/04/11 06:00 [medline]
PHST- 2017/04/28 06:00 [entrez]
AID - 3753550 [pii]
AID - 10.1093/femsec/fix055 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2017 May 1;93(5). doi: 10.1093/femsec/fix055.