PMID- 28449998
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20181202
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 231
DP  - 2017 Sep 15
TI  - Milk protein isolate (MPI) as a source of dipeptidyl peptidase IV (DPP-IV) 
      inhibitory peptides.
PG  - 202-211
LID - S0308-8146(17)30518-6 [pii]
LID - 10.1016/j.foodchem.2017.03.123 [doi]
AB  - A multifactorial [temperature (40, 50 and 60 degrees C), hydrolysis time (60, 150 and 
      240min) and enzyme to substrate ratio (E:S; 1.0, 1.5 and 2.0%)] design of 
      experiments (DOE) was used to optimise the release of dipeptidyl peptidase IV 
      (DPP-IV) inhibitory peptides during hydrolysis of bovine milk protein isolate 
      (MPI) with Neutrase 0.8L, yielding 15 hydrolysates (H1-H15). Variation in 
      temperature and time had a significant effect on DPP-IV inhibitory properties 
      (p<0.05) in contrast with E:S (p>0.05). The DPP-IV half maximal inhibitory 
      concentration (IC(50)) of H4, a potent sample, was maintained following simulated 
      gastrointestinal digestion (SGID, DPP-IV IC(50)=0.60+/-0.06vs. 0.58+/-0.09mgml(-1), 
      p>0.05). Several peptides with DPP-IV inhibitory features or known activity were 
      identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) within 
      the hydrolysates. MPI hydrolysates may have potential for use as dietary 
      ingredients with serum glucose lowering activity in humans.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Nongonierma, Alice B
AU  - Nongonierma AB
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland.
FAU - Lalmahomed, Mehdeeyah
AU  - Lalmahomed M
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland.
FAU - Paolella, Sara
AU  - Paolella S
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland.
FAU - FitzGerald, Richard J
AU  - FitzGerald RJ
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland. 
      Electronic address: dick.fitzgerald@ul.ie.
LA  - eng
PT  - Journal Article
DEP - 20170323
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Dipeptidyl-Peptidase IV Inhibitors)
RN  - 0 (Milk Proteins)
RN  - 0 (Peptides)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Dipeptidyl Peptidase 4
MH  - Dipeptidyl-Peptidase IV Inhibitors
MH  - Humans
MH  - *Milk Proteins
MH  - Peptides
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Bioactive peptides
OT  - Dipeptidyl peptidase IV inhibition
OT  - Milk protein isolate
OT  - Response surface methodology
EDAT- 2017/04/30 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/04/29 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/03/06 00:00 [revised]
PHST- 2017/03/22 00:00 [accepted]
PHST- 2017/04/29 06:00 [entrez]
PHST- 2017/04/30 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
AID - S0308-8146(17)30518-6 [pii]
AID - 10.1016/j.foodchem.2017.03.123 [doi]
PST - ppublish
SO  - Food Chem. 2017 Sep 15;231:202-211. doi: 10.1016/j.foodchem.2017.03.123. Epub 
      2017 Mar 23.