PMID- 28451399
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2049-9434 (Print)
IS  - 2049-9442 (Electronic)
IS  - 2049-9434 (Linking)
VI  - 6
IP  - 3
DP  - 2017 Mar
TI  - Preliminary evaluation of circulating microRNAs as potential biomarkers in 
      paracoccidioidomycosis.
PG  - 353-357
LID - 10.3892/br.2017.849 [doi]
AB  - MicroRNAs (miRNAs) are small RNAs (length, 19-24 nucleotides) that regulate gene 
      expression by either mRNA degradation or translational inhibition of proteins. 
      Circulating miRNAs, which are extremely stable and protected from RNAse-mediated 
      degradation in body fluids, have appeared as candidate biomarkers for numerous 
      diseases. However, little is known about circulating miRNAs in fungal infections. 
      Paracoccidioidomycosis (PCM) is caused by the Paracoccidioides species, and is 
      endemic in Central and South America, with predominance in adult male workers 
      from rural areas. The current study aimed to identify a serum miRNA expression 
      profile that could serve as a novel diagnostic biomarker for PCM. Total RNA was 
      isolated and the levels of circulating miRNAs were compared between patients with 
      PCM and healthy control subjects using reverse transcription-quantitative 
      polymerase chain reaction. Bioinformatic analysis was used to evaluate the 
      potential roles of these miRNAs in PCM. Eight miRNAs were differentially 
      expressed in serum samples from patients with PCM. These miRNAs are associated 
      with apoptosis and immune response. The identified miRNAs facilitate with 
      understanding the regulatory mechanisms involved in the host-parasite interaction 
      of PCM. Furthermore, considering that the diagnosis of PCM presents difficulties, 
      these miRNAs may serve as novel biomarkers for this disease.
FAU - De Lacorte Singulani, Junya
AU  - De Lacorte Singulani J
AD  - Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo 
      State University (UNESP), Araraquara, Sao Paulo 14800-901, Brazil.
FAU - De Fatima Da Silva, Julhiany
AU  - De Fatima Da Silva J
AD  - Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo 
      State University (UNESP), Araraquara, Sao Paulo 14800-901, Brazil.
FAU - Gullo, Fernanda Patricia
AU  - Gullo FP
AD  - Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo 
      State University (UNESP), Araraquara, Sao Paulo 14800-901, Brazil.
FAU - Costa, Marina Celia
AU  - Costa MC
AD  - Faculty of Medicine, Institute of Molecular Medicine, University of Lisbon, 
      1649-004 Lisbon, Portugal.
FAU - Fusco-Almeida, Ana Marisa
AU  - Fusco-Almeida AM
AD  - Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo 
      State University (UNESP), Araraquara, Sao Paulo 14800-901, Brazil.
FAU - Enguita, Francisco Javier
AU  - Enguita FJ
AD  - Faculty of Medicine, Institute of Molecular Medicine, University of Lisbon, 
      1649-004 Lisbon, Portugal.
FAU - Mendes-Giannini, Maria Jose Soares
AU  - Mendes-Giannini MJS
AD  - Department of Clinical Analysis, School of Pharmaceutical Sciences, Sao Paulo 
      State University (UNESP), Araraquara, Sao Paulo 14800-901, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170126
PL  - England
TA  - Biomed Rep
JT  - Biomedical reports
JID - 101613227
PMC - PMC5403430
OTO - NOTNLM
OT  - Paracoccidioides spp.
OT  - biological function
OT  - biomarker
OT  - microRNA
OT  - serum
EDAT- 2017/04/30 06:00
MHDA- 2017/04/30 06:01
PMCR- 2017/01/26
CRDT- 2017/04/29 06:00
PHST- 2016/09/26 00:00 [received]
PHST- 2017/01/09 00:00 [accepted]
PHST- 2017/04/29 06:00 [entrez]
PHST- 2017/04/30 06:00 [pubmed]
PHST- 2017/04/30 06:01 [medline]
PHST- 2017/01/26 00:00 [pmc-release]
AID - BR-0-0-849 [pii]
AID - 10.3892/br.2017.849 [doi]
PST - ppublish
SO  - Biomed Rep. 2017 Mar;6(3):353-357. doi: 10.3892/br.2017.849. Epub 2017 Jan 26.