PMID- 28453462
OWN - NLM
STAT- MEDLINE
DCOM- 20180426
LR  - 20180426
IS  - 1875-8592 (Electronic)
IS  - 1574-0153 (Linking)
VI  - 19
IP  - 3
DP  - 2017 Jul 4
TI  - Up-regulated miR-221 expression as a molecular diagnostic marker in laryngeal 
      squamous cell carcinoma and its correlation with Apaf-1 expression.
PG  - 279-287
LID - 10.3233/CBM-160444 [doi]
AB  - BACKGROUND: Despite the improvement in the diagnosis and the management of 
      laryngeal squamous cell carcinoma (LSCC), many patients with advanced-stage have 
      poor prognosis in the form of recurrence, metastasis or death. So, recognition of 
      new molecular markers would facilitate the development of targeted therapies. 
      OBJECTIVES: To investigate miR-221 expression in LSCC and its possible 
      correlation to apoptotic protease activating factor-1 (Apaf-1). Also, we aimed to 
      investigate the association between miR-221 and Apaf-1 expressions and the 
      clinicopathological features of LSCC. METHODS: We investigated the expression of 
      miR-221 (by qRT-PCR) and Apaf-1 (by qRT-PCR and immunohistochemistry) in primary 
      LSCC and adjacent normal tissues. RESULTS: We found significant up-regulation of 
      miR-221 and significant down-regulation of Apaf-1 expression in LSCC tissues 
      compared to normal nearby laryngeal tissues. In addition, significant 
      associations between up-regulated miR-221 and down-regulated Apaf-1 expressions 
      and clinical stage and lymph node (LN) metastasis (P< 0.001 for each) were found. 
      Furthermore, there was a negative correlation between miR-221 gene expression and 
      Apaf-1 gene expression (r=-0.73, P< 0.001). CONCLUSION: miR-221 can be considered 
      as a diagnostic marker in LSCC and Apaf-1 may be considered as a possible target 
      of miR-221.
FAU - Hussein, Samia
AU  - Hussein S
AD  - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, 
      Zagazig University, Zagazig, Egypt.
FAU - Mosaad, Hala
AU  - Mosaad H
AD  - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, 
      Zagazig University, Zagazig, Egypt.
FAU - Rashed, Hayam E
AU  - Rashed HE
AD  - Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
FAU - El-Anwar, Mohammad W
AU  - El-Anwar MW
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Faculty of Medicine, 
      Zagazig University, Zagazig, Egypt.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Cancer Biomark
JT  - Cancer biomarkers : section A of Disease markers
JID - 101256509
RN  - 0 (APAF1 protein, human)
RN  - 0 (Apoptotic Protease-Activating Factor 1)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN221 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptotic Protease-Activating Factor 1/*biosynthesis/genetics/metabolism
MH  - Biomarkers, Tumor
MH  - Carcinoma, Squamous Cell/*diagnosis/pathology
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*diagnosis/pathology
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Prognosis
MH  - Up-Regulation
OTO - NOTNLM
OT  - Apaf-1
OT  - Laryngeal squamous cell carcinoma
OT  - miR-221
EDAT- 2017/04/30 06:00
MHDA- 2018/04/27 06:00
CRDT- 2017/04/29 06:00
PHST- 2017/04/30 06:00 [pubmed]
PHST- 2018/04/27 06:00 [medline]
PHST- 2017/04/29 06:00 [entrez]
AID - CBM160444 [pii]
AID - 10.3233/CBM-160444 [doi]
PST - ppublish
SO  - Cancer Biomark. 2017 Jul 4;19(3):279-287. doi: 10.3233/CBM-160444.