PMID- 28454468
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 13
IP  - 4
DP  - 2017 Apr
TI  - Ethanolic extract of Thevetia peruviana flowers enhances TNF-alpha and TRAIL-induced 
      apoptosis of human cervical cancer cells via intrinsic and extrinsic pathways.
PG  - 2791-2798
LID - 10.3892/ol.2017.5748 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) and TNF-related apoptosis-inducing ligand (TRAIL) 
      are promising candidates for cancer treatment due to their ability to induce 
      apoptosis through death receptor stimulation. However, their usage may be limited 
      due to the resistance of cancer cells to TNF-alpha- and TRAIL-induced apoptosis. 
      Currently, there is interest in screening for natural products that can sensitize 
      cancer cells to TNF-alpha- and TRAIL-induced apoptosis for their use in combination 
      with TNF-alpha or TRAIL. It was previously reported that the bark extract of Thevetia 
      peruviana showed a reversal effect on TRAIL-resistance in human gastric 
      adenocarcinoma cell lines. In the present study, the effects of the ethanolic 
      extract of T. peruviana flowers on TNF-alpha- and TRAIL-induced apoptosis of human 
      cervical cancer HeLa cells were investigated in vitro by determining cell 
      viability and apoptosis using a WST-1 cell proliferation assay and immunoblot 
      analysis, respectively. The ethanolic extract of T. peruviana flowers promoted 
      TNF-alpha and TRAIL-mediated cell death through the activation of the caspase 
      cascade, poly(ADP-ribose) polymerase and BH3-interacting domain death agonist 
      cleavage. Combined treatment using the extract plus TNF-alpha resulted in 
      downregulation of anti-apoptotic protein, including myeloid cell leukemia 
      sequence-1, B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis 
      protein and survivin, while the combined treatment with TRAIL downregulated 
      Bcl-XL. Thus, the ethanolic extract of T. peruviana flowers has potential in 
      sensitizing the TNF-alpha- and TRAIL-induced apoptosis of HeLa cells via the 
      intrinsic and extrinsic pathways.
FAU - Managit, Chittima
AU  - Managit C
AD  - Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot 
      University, Nakhonnayok 26120, Thailand.
FAU - Sakurai, Hiroaki
AU  - Sakurai H
AD  - Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, Toyama 930-0194, Japan.
FAU - Saiki, Ikuo
AU  - Saiki I
AD  - Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of 
      Toyama, Toyama 930-0194, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170217
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5403174
OTO - NOTNLM
OT  - B-cell lymphoma-extra large
OT  - HeLa cells
OT  - anti-apoptotic protein
OT  - cardiac glycosides
OT  - cervical cancer
OT  - death receptor
OT  - plant extract
EDAT- 2017/04/30 06:00
MHDA- 2017/04/30 06:01
PMCR- 2017/02/17
CRDT- 2017/04/30 06:00
PHST- 2015/06/24 00:00 [received]
PHST- 2016/09/15 00:00 [accepted]
PHST- 2017/04/30 06:00 [entrez]
PHST- 2017/04/30 06:00 [pubmed]
PHST- 2017/04/30 06:01 [medline]
PHST- 2017/02/17 00:00 [pmc-release]
AID - OL-0-0-5748 [pii]
AID - 10.3892/ol.2017.5748 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Apr;13(4):2791-2798. doi: 10.3892/ol.2017.5748. Epub 2017 Feb 
      17.