PMID- 28455412
OWN - NLM
STAT- MEDLINE
DCOM- 20180509
LR  - 20181113
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 130
IP  - 12
DP  - 2017 Jun 15
TI  - In vivo reprogramming of non-mammary cells to an epithelial cell fate is 
      independent of amphiregulin signaling.
PG  - 2018-2025
LID - 10.1242/jcs.200030 [doi]
AB  - Amphiregulin (AREG)(-/-) mice demonstrate impaired mammary development and form 
      only rudimentary ductal epithelial trees; however, AREG(-/-) glands are still 
      capable of undergoing alveologenesis and lactogenesis during pregnancy. 
      Transplantation of AREG(-/-) mammary epithelial cells into cleared mouse mammary 
      fat pads results in a diminished capacity for epithelial growth ( approximately 15%) as 
      compared to that of wild-type mammary epithelial cells. To determine whether 
      estrogen receptor alpha (ERalpha, also known as ESR1) and/or AREG signaling were 
      necessary for non-mammary cell redirection, we inoculated either ERalpha(-/-) or 
      AREG(-/-) mammary cells with non-mammary progenitor cells (WAP-Cre/Rosa26LacZ+ 
      male testicular cells or GFP-positive embryonic neuronal stem cells). ERalpha(-/-) 
      cells possessed a limited ability to grow or reprogram non-mammary cells in 
      transplanted mammary fat pads. AREG(-/-) mammary cells were capable of 
      redirecting both types of non-mammary cell populations to mammary phenotypes in 
      regenerating mammary outgrowths. Transplantation of fragments from 
      AREG-reprogrammed chimeric outgrowths resulted in secondary outgrowths in six out 
      of ten fat pads, demonstrating the self-renewing capacity of the redirected 
      non-mammary cells to contribute new progeny to chimeric outgrowths. Nestin was 
      detected at the leading edges of developing alveoli, suggesting that its 
      expression may be essential for lobular expansion.
CI  - (c) 2017. Published by The Company of Biologists Ltd.
FAU - George, Andrea L
AU  - George AL
AD  - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, 
      National Institutes of Health, Bethesda, MD, USA.
FAU - Boulanger, Corinne A
AU  - Boulanger CA
AD  - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, 
      National Institutes of Health, Bethesda, MD, USA.
FAU - Anderson, Lisa H
AU  - Anderson LH
AUID- ORCID: 0000-0001-9541-1950
AD  - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, 
      National Institutes of Health, Bethesda, MD, USA.
FAU - Cagnet, Stephanie
AU  - Cagnet S
AD  - Ecole Polytechnique Federale de Lausanne (EPFL), ISREC-Swiss Institute for 
      Experimental Research, SV.832 Station 19, Lausanne CH-1015, Switzerland.
FAU - Brisken, Cathrin
AU  - Brisken C
AD  - Ecole Polytechnique Federale de Lausanne (EPFL), ISREC-Swiss Institute for 
      Experimental Research, SV.832 Station 19, Lausanne CH-1015, Switzerland.
FAU - Smith, Gilbert H
AU  - Smith GH
AUID- ORCID: 0000-0002-1908-5425
AD  - Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, 
      National Institutes of Health, Bethesda, MD, USA smithg@mail.nih.gov.
LA  - eng
PT  - Journal Article
DEP - 20170428
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amphiregulin/*genetics
MH  - Animals
MH  - Cell Differentiation
MH  - *Cell Lineage
MH  - Cell Proliferation
MH  - Cell Transplantation
MH  - *Cellular Reprogramming
MH  - Cerebral Cortex/embryology
MH  - Embryonic Stem Cells/cytology
MH  - Epithelial Cells/*cytology
MH  - Estrogen Receptor alpha/genetics
MH  - Estrogens/metabolism
MH  - Female
MH  - Green Fluorescent Proteins/metabolism
MH  - Male
MH  - Mammary Glands, Animal/cytology
MH  - Mice
MH  - Mice, Nude
MH  - Mice, Transgenic
MH  - Neural Stem Cells/cytology
MH  - Pregnancy
MH  - *Signal Transduction
MH  - Spermatozoa/metabolism
MH  - Testis/metabolism
PMC - PMC5482980
OTO - NOTNLM
OT  - Amphiregulin
OT  - Estrogen
OT  - Mammary
OT  - Reprogramming
OT  - Stem cells
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2017/04/30 06:00
MHDA- 2018/05/10 06:00
PMCR- 2018/06/15
CRDT- 2017/04/30 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/04/30 06:00 [pubmed]
PHST- 2018/05/10 06:00 [medline]
PHST- 2017/04/30 06:00 [entrez]
PHST- 2018/06/15 00:00 [pmc-release]
AID - jcs.200030 [pii]
AID - JCS200030 [pii]
AID - 10.1242/jcs.200030 [doi]
PST - ppublish
SO  - J Cell Sci. 2017 Jun 15;130(12):2018-2025. doi: 10.1242/jcs.200030. Epub 2017 Apr 
      28.