PMID- 28457017
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20231112
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 61
IP  - 9
DP  - 2017 Sep
TI  - Signatures of anthocyanin metabolites identified in humans inhibit biomarkers of 
      vascular inflammation in human endothelial cells.
LID - 10.1002/mnfr.201700053 [doi]
LID - 1700053
AB  - SCOPE: The physiological relevance of contemporary cell culture studies is often 
      perplexing, given the use of unmetabolized phytochemicals at supraphysiological 
      concentrations. We investigated the activity of physiologically relevant 
      anthocyanin metabolite signatures, derived from a previous pharmacokinetics study 
      of 500 mg (13) C(5) -cyanidin-3-glucoside in eight healthy participants, on 
      soluble vascular adhesion molecule-1 (VCAM-1) and interleukin-6 (IL-6) in human 
      endothelial cells. METHODS AND RESULTS: Signatures of peak metabolites 
      (previously identified at 1, 6, and 24 h post-bolus) were reproduced using pure 
      standards and effects were investigated across concentrations ten-fold lower and 
      higher than observed mean (<5 muM) serum levels. Tumor necrosis factor-alpha 
      (TNF-alpha)-stimulated VCAM-1 was reduced in response to all treatments, with maximal 
      effects observed for the 6 and 24 h profiles. Profiles tested at ten-fold below 
      mean serum concentrations (0.19-0.44 muM) remained active. IL-6 was reduced in 
      response to 1, 6, and 24 h profiles, with maximal effects observed for 6 h and 
      24 h profiles at concentrations above 2 muM. Protein responses were reflected by 
      reductions in VCAM-1 and IL-6 mRNA, however there was no effect on phosphorylated 
      NFkappaB-p65 expression. CONCLUSION: Signatures of anthocyanin metabolites following 
      dietary consumption reduce VCAM-1 and IL-6 production, providing evidence of 
      physiologically relevant biological activity.
CI  - (c) 2017 The Authors. Molecular Nutrition & Food Research published by WILEY-VCH 
      Verlag GmbH & Co. KGaA, Weinheim.
FAU - Warner, Emily F
AU  - Warner EF
AD  - Department of Nutrition, Norwich Medical School, Bob Champion Research and 
      Education Building, University of East Anglia, Norwich, UK.
AD  - School of Pharmacy, University of East Anglia, Norwich, UK.
FAU - Smith, Michael J
AU  - Smith MJ
AD  - Department of Nutrition, Norwich Medical School, Bob Champion Research and 
      Education Building, University of East Anglia, Norwich, UK.
AD  - School of Biological Sciences, University of East Anglia, Norwich, UK.
FAU - Zhang, Qingzhi
AU  - Zhang Q
AD  - School of Chemistry, University of St. Andrews, Fife, Scotland, UK.
FAU - Raheem, K Saki
AU  - Raheem KS
AD  - School of Chemistry, University of St. Andrews, Fife, Scotland, UK.
AD  - Department of Life Sciences, Faculty of Science and Technology, University of 
      Westminster, London, UK.
FAU - O'Hagan, David
AU  - O'Hagan D
AD  - School of Chemistry, University of St. Andrews, Fife, Scotland, UK.
FAU - O'Connell, Maria A
AU  - O'Connell MA
AD  - School of Pharmacy, University of East Anglia, Norwich, UK.
FAU - Kay, Colin D
AU  - Kay CD
AD  - Department of Nutrition, Norwich Medical School, Bob Champion Research and 
      Education Building, University of East Anglia, Norwich, UK.
AD  - Food Bioprocessing & Nutrition Sciences, Plants for Human Health Institute, North 
      Carolina State University, Kannapolis, NC, USA.
LA  - eng
GR  - BB/H004726/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/H004963/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/H00503X/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
DEP - 20170609
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Anthocyanins)
RN  - 0 (Biomarkers)
RN  - 0 (Glucosides)
RN  - 0 (Interleukin-6)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (cyanidin-3-O-beta-glucopyranoside)
SB  - IM
MH  - Anthocyanins/*metabolism/pharmacology
MH  - Biomarkers
MH  - Cells, Cultured
MH  - Endothelial Cells/*drug effects
MH  - Glucosides/*pharmacology
MH  - Humans
MH  - Interleukin-6/analysis/*antagonists & inhibitors/genetics
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/analysis/*antagonists & inhibitors/genetics
PMC - PMC5600085
OTO - NOTNLM
OT  - Adhesion
OT  - Anthocyanin
OT  - Inflammation
OT  - Metabolism
EDAT- 2017/05/01 06:00
MHDA- 2018/07/24 06:00
PMCR- 2017/09/15
CRDT- 2017/05/01 06:00
PHST- 2017/01/15 00:00 [received]
PHST- 2017/03/15 00:00 [revised]
PHST- 2017/04/11 00:00 [accepted]
PHST- 2017/05/01 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PHST- 2017/05/01 06:00 [entrez]
PHST- 2017/09/15 00:00 [pmc-release]
AID - MNFR2920 [pii]
AID - 10.1002/mnfr.201700053 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2017 Sep;61(9):1700053. doi: 10.1002/mnfr.201700053. Epub 2017 
      Jun 9.