PMID- 28463545
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20220408
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 24
IP  - 1-2
DP  - 2018 Jan
TI  - Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison 
      of Different Human Cell Sources.
PG  - 81-93
LID - 10.1089/ten.TEA.2016.0205 [doi]
AB  - Hydrogel and electrospun scaffold materials support cell attachment and neotissue 
      development and can be tuned to structurally and mechanically resemble native 
      extracellular matrix by altering either electrospun fiber or hydrogel properties. 
      In this study, we examined meniscus tissue generation from different human cell 
      sources including meniscus cells derived from vascular and avascular regions, 
      human bone marrow-derived mesenchymal stem cells, synovial cells, and cells from 
      the infrapatellar fat pad (IPFP). All cells were seeded onto aligned electrospun 
      collagen type I scaffolds and were optionally encapsulated in a tricomponent 
      hydrogel. Single or multilayered constructs were generated and cultivated in 
      defined medium with selected growth factors for 2 weeks. Cell viability, cell 
      morphology, and gene-expression profiles were monitored using confocal 
      microscopy, scanning electron microscopy, and quantitative polymerase chain 
      reaction (qPCR), respectively. Multilayered constructs were examined with 
      histology, immunohistochemistry, qPCR, and for tensile mechanical properties. For 
      all cell types, TGFbeta1 and TGFbeta3 treatment increased COL1A1, COMP, Tenascin C 
      (TNC), and Scleraxis (SCX) gene expression and deposition of collagen type I 
      protein. IPFP cells generated meniscus-like tissues with higher meniscogenic gene 
      expression, mechanical properties, and better cell distribution compared to other 
      cell types studied. We show proof of concept that electrospun collagen scaffolds 
      support neotissue formation and IPFP cells have potential for use in cell-based 
      meniscus regeneration strategies.
FAU - Baek, Jihye
AU  - Baek J
AD  - 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La 
      Jolla, California.
AD  - 2 Department of Material Science and Engineering, University of California , San 
      Diego, La Jolla, California.
FAU - Sovani, Sujata
AU  - Sovani S
AD  - 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La 
      Jolla, California.
FAU - Choi, Wonchul
AU  - Choi W
AD  - 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La 
      Jolla, California.
FAU - Jin, Sungho
AU  - Jin S
AD  - 2 Department of Material Science and Engineering, University of California , San 
      Diego, La Jolla, California.
FAU - Grogan, Shawn P
AU  - Grogan SP
AD  - 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La 
      Jolla, California.
FAU - D'Lima, Darryl D
AU  - D'Lima DD
AD  - 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La 
      Jolla, California.
LA  - eng
GR  - P01 AG007996/AG/NIA NIH HHS/United States
GR  - UL1 RR025774/RR/NCRR NIH HHS/United States
GR  - P40 RR017447/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170613
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adult
MH  - Cell Count
MH  - Cells, Cultured
MH  - Collagen/*chemistry
MH  - Female
MH  - Humans
MH  - Male
MH  - Meniscus/*cytology
MH  - Microscopy, Electron, Scanning
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
PMC - PMC5770095
OTO - NOTNLM
OT  - adult stem cells
OT  - biomimetic materials
OT  - meniscus
COIS- No competing financial interests exist.
EDAT- 2017/05/04 06:00
MHDA- 2018/11/06 06:00
PMCR- 2019/01/01
CRDT- 2017/05/03 06:00
PHST- 2017/05/04 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2017/05/03 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2016.0205 [pii]
AID - 10.1089/ten.TEA.2016.0205 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2018 Jan;24(1-2):81-93. doi: 10.1089/ten.TEA.2016.0205. Epub 
      2017 Jun 13.