PMID- 2846394
OWN - NLM
STAT- MEDLINE
DCOM- 19881222
LR  - 20220311
IS  - 0301-4681 (Print)
IS  - 0301-4681 (Linking)
VI  - 38
IP  - 1
DP  - 1988 Jun
TI  - Human bronchial epithelial cells with integrated SV40 virus T antigen genes 
      retain the ability to undergo squamous differentiation.
PG  - 60-6
AB  - Human bronchial epithelial cells transformed by either DNA virus infection (SV40 
      or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen 
      gene were studied for their ability to undergo squamous differentiation when 
      exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth 
      factor-beta 1 (TGF-beta 1), or fetal bovine serum (FBS), agents that induce the 
      squamous differentiation of normal human bronchial epithelial cells. Squamous 
      differentiation occurred in all ten T-antigen-positive cell cultures when they 
      were exposed to either FBS or TGF-beta 1, but none differentiated when exposed to 
      TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of 
      which was induced to undergo squamous differentiation by FBS, and a second that 
      failed to undergo squamous differentiation and was mitogenically stimulated when 
      exposed to serum. These phenotypically different subclones provide a new in vitro 
      cellular system for delineating the mechanism(s) of human bronchial epithelial 
      cell squamous differentiation in response to FBS or TGF-beta 1.
FAU - Ke, Y
AU  - Ke Y
AD  - Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
FAU - Reddel, R R
AU  - Reddel RR
FAU - Gerwin, B I
AU  - Gerwin BI
FAU - Miyashita, M
AU  - Miyashita M
FAU - McMenamin, M
AU  - McMenamin M
FAU - Lechner, J F
AU  - Lechner JF
FAU - Harris, C C
AU  - Harris CC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (Antigens, Viral)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 64604-09-7 (12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Antigens, Viral/*metabolism
MH  - Bronchi/*cytology/drug effects/immunology
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Humans
MH  - Parainfluenza Virus 1, Human/*immunology
MH  - Serum Albumin, Bovine/pharmacology
MH  - Tetradecanoylphorbol Acetate/analogs & derivatives/pharmacology
MH  - Transforming Growth Factors/pharmacology
MH  - Tumor Cells, Cultured/cytology/drug effects/*immunology
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
AID - S0301-4681(11)60454-4 [pii]
AID - 10.1111/j.1432-0436.1988.tb00592.x [doi]
PST - ppublish
SO  - Differentiation. 1988 Jun;38(1):60-6. doi: 10.1111/j.1432-0436.1988.tb00592.x.